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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제1호
- 발행연도
- 2008.1
- 수록면
- 118 - 123 (6page)
이용수
초록· 키워드
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A new method for antimicrobial susceptibility testing of vitrocultured
bacteria on an ordinary fluorescence spectrometer
was developed. The viable bacteria reduced 3-(4,5-
dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide
(MTT) to produce insoluble particles that displayed intense
resonance scattering light. The assay showed a linear
relationship between the number of viable bacteria and the
intensity of resonance scattering light. Dead bacteria were
unable to reduce MTT. Methicillin-resistant Staphylococcus
aureus exposed to flavonoids from Marchantia convoluta
showed a flavonoids concentration-dependent inhibition
of the ability to reduce MTT. In the assay, less than 12 h
was required to attain susceptibility results and fewer
bacteria were utilized than in traditional methods. The
RLS technique could, in combination with the MTT assay,
be a rapid and sensitive measuring method to determine
the in vitro activity of new antimicrobials.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Aslan, K. / 2005 / Angular-ratiometric plasmon-resonance based light scattering for bioaffinity sensing. J. Am. Chem / Soc 127 : 12115 ~ 12121
Aslan, K. / 2005 / Nanogold plasmon resonance-based glucose sensing. 2. Wavelengthratiometric resonance light scattering. Anal / Chem 77 : 2007 ~ 2014
Aziz, D. M. / 2005 / Application of MTT reduction assay to evaluate equine sperm viability / Theriogenology 64 : 1350 ~ 1356
Aziz, D. M. / 2006 / Assessment of bovine sperm viability by MTT reduction assay. Anim. Reprod / Sci 92 : 1 ~ 8
Chen, Z. G. / 2005 / Determination of nucleic acids based on their resonance light scattering enhancement effect on metalloporphyrin derivatives. Microchim / Acta 150 : 34 ~
Aslan, K. / 2005 / Nanogold plasmon resonance-based glucose sensing. 2. Wavelengthratiometric resonance light scattering. Anal / Chem 77 : 2007 ~ 2014
Aziz, D. M. / 2005 / Application of MTT reduction assay to evaluate equine sperm viability / Theriogenology 64 : 1350 ~ 1356
Aziz, D. M. / 2006 / Assessment of bovine sperm viability by MTT reduction assay. Anim. Reprod / Sci 92 : 1 ~ 8
Chen, Z. G. / 2005 / Determination of nucleic acids based on their resonance light scattering enhancement effect on metalloporphyrin derivatives. Microchim / Acta 150 : 34 ~
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