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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.1
- 수록면
- 601 - 607 (7page)
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초록· 키워드
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Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Here, we report the identification of an alternatively-spliced variant of nanog. This variant lacked a stretch of amino acids (residues 168-183) located between the HD and tryptophan-repeat (WR) of the previously-reported full length sequence, suggesting that the deleted flexibility of the C-terminal transactivation domain relative to the DNA binding domain. Expression of mRNA encoding the splice variant, designated as nanog-delta 48, was much lower than that of the full length version in human ES cells. The ratio of nanog-delta 48 transcript to full length transcript increased, however, in multipotent adult progenitor cells. EMSA analysis revealed that both forms of Nanog were able to bind a nanog binding sequence with roughly the same affinity. A reporter plasmid assay also showed that both variants of nanog modestly repressed transactivation of gata-4, whose expression is proposed to be inhibited by nanog, with comparable primary structure and expression pattern in various stem cells, the alternatively-spliced variant of Nanog has similar activity to that of the full length version.
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참고문헌 (14)
참고문헌 신청
/ / Cantley LC.A motif-based profile scanning approach for genome-wideprediction of signaling pathways. Nature 2001 nia s : 348 ~ 53
/ / KimJH. Identification of a putative transactivation domain inhuman Nanog. Exp Mol Med 2005 37 : 250 ~ 4
/ / Lee YH. Implication ofEgr-1 in trigluoperazine-induced growth inhibition in humanU87MG glioma cells. Exp Mol Med 2004 : 380 ~ 6
/ / Pei DQ. Identification of two distinct transactivationdomains in the pluripotency sustaining factor Nanog. CellRes 2003 13 : 499 ~ 502
/ / Pei DQ. The stem cell pluripotency factor nanogactivates transcription with two unusually potent subdomainsat its c-terminus. J Biol Chem 2005 transcriptional profiling
/ / KimJH. Identification of a putative transactivation domain inhuman Nanog. Exp Mol Med 2005 37 : 250 ~ 4
/ / Lee YH. Implication ofEgr-1 in trigluoperazine-induced growth inhibition in humanU87MG glioma cells. Exp Mol Med 2004 : 380 ~ 6
/ / Pei DQ. Identification of two distinct transactivationdomains in the pluripotency sustaining factor Nanog. CellRes 2003 13 : 499 ~ 502
/ / Pei DQ. The stem cell pluripotency factor nanogactivates transcription with two unusually potent subdomainsat its c-terminus. J Biol Chem 2005 transcriptional profiling
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