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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.1
- 수록면
- 250 - 254 (5page)
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초록· 키워드
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Nanog is a newly identified divergent homeodo-main protein that directs the infinite propagation and sustains the pluripotency of embryonic stem cels. It has been reported that murine Nanog has two potent transactivation domains in N-terminal and C-terminal regions. Human Nanog (hNanog) to the open reading frame and homeodomain of murine Nanog, respectively. However, the func-tional domains and molecular mechanisms of hNanog are poorly understood. In this study, for the first time, we presented that only C-terminus of hNanog contains a potent transactivation do-main. Based on the amino acid sequences of homeobox domain, we roughly divided hNanog open reading frame into the thre regions such as N-terminal, homeodomain and C-terminal re-gions and constructed either the fusion pro-binding domain or the context of native hNanog protein. Reporter asays by using reporter pla-mid containing Gal4 or Nanog binding site revealed that the only C-terminal region exhibited the significant fold induction of transactivation. However, interestingly, there was no significant activation through N-terminal region unlike mu-rine Nanog, suggesting that C-terminal region may have more critical roles in the transcriptional activation of target genes. Taken together, the finding of a putative transactivation domain in hNanog may contribute to the further understand-downstream genes involved in self-renewal and pluripotency of human stem cels.
목차
등록된 정보가 없습니다.
참고문헌 (13)
참고문헌 신청
/ / A proline-richtranscriptional activation domain in murine HOXD-4 : 376 ~ 4 82
/ / Embryonic stem cell lines from human blastocysts somaticdifferentiation in vitro 18 (22) : 399 ~ 404
/ / Identification of two distinct transactivationdomains in the pluripotency sustaining factor nanog : 499 ~ 502
/ / Identificationand functional characterization of a Smad bindingelement in the JunB promoter that acts as a transforminggrowth factor-β : 21145 ~ 52
/ / The stem cell pluripotency factor nanogactivates transcription with two unusually potent subdomainsat its C-terminus : 1401 ~ 7
/ / Embryonic stem cell lines from human blastocysts somaticdifferentiation in vitro 18 (22) : 399 ~ 404
/ / Identification of two distinct transactivationdomains in the pluripotency sustaining factor nanog : 499 ~ 502
/ / Identificationand functional characterization of a Smad bindingelement in the JunB promoter that acts as a transforminggrowth factor-β : 21145 ~ 52
/ / The stem cell pluripotency factor nanogactivates transcription with two unusually potent subdomainsat its C-terminus : 1401 ~ 7
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