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Pathogenesis of the abdominal aortic aneurysm has been atributed to neovascularization of the aortic wall. However, it is not clear whether angiogenesis persists in the aneurysm. In sec-tions of aneurysms, we determined the imuno-histochemical distributions of the αvβ3 integrin, tenascin and endothelial nitric oxide synthase (eNOS), which are markers respectively, of an-giogenesis, matrix remodeling and vasoregula-tory function. In addition, we used reverse trans-cription folowed by in situ PCR, to determine the distribution of αv mRNA. Al aneurysm spec-imens exhibited extensive increases of wal vas-cularization as compared with the control aortic wall, and showed the presence of perivascular inflamatory exudates containing macrophages and lymphocytes. The neovascularization con-sisted of thick-waled vessels in the media and majority of vessels stained positively for the αvβ3 antigen and eNOS. Tenascin was deposited as bands that circumscribed thick-waled ves-sels. The distribution of av mRNA was extensive and was positive even in those vessels that failed to stain for the αvβ3 protein. No staining was evident in control aortas for the αvβ3 anti-gen, tenascin or αv mRNA. The upregulation of αv αvβ3 integrin in blod vessels surounded by a matrix expresing tenascin, indicates that angiogenesis is an ongoing pro-cess in the mature aortic aneurysm.

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