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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 377 - 386 (10page)
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초록· 키워드
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Tumor associated microtubule associated protein
(TMAP), also known as cytoskeleton associated protein
2 (CKAP2) is a mitotic spindle-associated protein
whose expression is cell cycle-regulated and also frequently
deregulated in cancer cells. Two monoclonal
antibodies (mAbs) against TMAP/CKAP2 were produced:
B-1-13 and D-12-3. Interestingly, the reactivity
of mAb D-12-3 to TMAP/CKAP2 was markedly decreased
specifically in mitotic cell lysate. The epitope
mapping study showed that mAb D-12-3 recognizes
the amino acid sequence between 569 and 625 and that
phosphorylation at T596 completely abolishes the reactivity
of the antibody, suggesting that the differential
reactivity originates from the phosphorylation status
at T596. Immunofluorescence staining showed that
mAb D-12-3 fails to detect TMAP/CKAP2 in mitotic cells
between prophase and metaphase, but the staining becomes
evident again in anaphase, suggesting that phosphorylation at T596 occurs transiently during early
phases of mitosis. These results suggest that the cellular
functions of TMAP/CKAP2 might be regulated by
timely phosphorylation and dephosphorylation during
the course of mitosis.
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참고문헌 (23)
참고문헌 신청
Bae CD / 2003 / Up-regulation of cytoskeletalassociated protein 2 in primary human gastric adenocarcinomas / J Cancer Res Clin Oncol 129 : 621 ~ 30
Carmena W / 2003 / The cellular geography of aurora kinases / Nat Rev Mol Cell Biol 4 : 842 ~ 54
Eichmuller S / 2001 / Serological detection of cutaneous T-cell lymphoma-associated antigens / Proc Natl Acad Sci USA 98 : 629 ~ 34
Hong KU / 2007 / Functional importance of the anaphase-promoting complex- Cdh1-mediated degradation of TMAP/CKAP2 in regulation of spindle function and cytokinesis / Mol Cell
Iyer VR / 1999 / The transcriptional program in the response of human fibroblasts to serum / Science 283 : 83 ~ 7
Carmena W / 2003 / The cellular geography of aurora kinases / Nat Rev Mol Cell Biol 4 : 842 ~ 54
Eichmuller S / 2001 / Serological detection of cutaneous T-cell lymphoma-associated antigens / Proc Natl Acad Sci USA 98 : 629 ~ 34
Hong KU / 2007 / Functional importance of the anaphase-promoting complex- Cdh1-mediated degradation of TMAP/CKAP2 in regulation of spindle function and cytokinesis / Mol Cell
Iyer VR / 1999 / The transcriptional program in the response of human fibroblasts to serum / Science 283 : 83 ~ 7
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