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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 721 - 727 (7page)
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초록· 키워드
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Cyclooxygenase-2 (COX-2) is known to modulate bone metabolism, including bone formation and resorption.
Because cartilage serves as a template for endochondral bone formation and because cartilage development is initiated by the differentiation of mesenchymal cells into chondrocytes (Ahrens et al., 1977; Sandell and Adler, 1999; Solursh, 1989), it is of interest to know whether COX-2 expression affect chondrocyte differentiation. Therefore, we investigated the effects of COX-2 protein on differentiation in rabbit articular chondrocyte and chick limb bud mesenchymal cells.
Overexpression of COX-2 protein was induced by the COX-2 cDNA transfection. Ectopic expression of COX-2 was sufficient to causes dedifferentiation in articular chondrocytes as determined by the expression of type II collagen via Alcian blue staining and Western blot. Also, COX-2 overexpression caused suppression of SOX-9 expression, a major transcription factor that regulates type II collagen expression, as indicated by the Western blot and RT-PCR. We further examined ectopic expression of COX-2 in chondrifying mesenchymal cells. As expected, COX-2 cDNA transfection blocked cartilage nodule formation as determined by Alcian blue staining. Our results collectively suggest that COX-2 overexpression causes dedifferentiation in articular chondrocytes and inhibits chondrogenic differentiation of mesenchymal cells.
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참고문헌 (24)
참고문헌 신청
Abramson SB / 1999 / The role of COX-2 produced by cartilage in arthritis / Osteoarthritis Cartilage 7 : 380 ~ 1
Ahrens PB / 1977 / Stage-related capacity for limb chondrogenesis in cell culture / Dev Biol 60 : 69 ~ 82
Amin AR / 1998 / The role of nitric oxide in articular cartilage breakdown in osteoarthritis / Curr Opin Rheumatol 10 : 263 ~ 8
Amin AR / 2000 / COX-2, NO, and cartilage damage and repair / Curr Rheumatol Rep 2 : 447 ~ 53
Aulthouse AL / 1987 / The detection of a precartilage, blastoma-specific marker / Dev Biol 120 : 377 ~ 84
Ahrens PB / 1977 / Stage-related capacity for limb chondrogenesis in cell culture / Dev Biol 60 : 69 ~ 82
Amin AR / 1998 / The role of nitric oxide in articular cartilage breakdown in osteoarthritis / Curr Opin Rheumatol 10 : 263 ~ 8
Amin AR / 2000 / COX-2, NO, and cartilage damage and repair / Curr Rheumatol Rep 2 : 447 ~ 53
Aulthouse AL / 1987 / The detection of a precartilage, blastoma-specific marker / Dev Biol 120 : 377 ~ 84
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