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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2006.1
- 수록면
- 36 - 43 (8page)
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Serine protease activity of high temperature
requrement 2 (HtrA2) is essential for promoting cell
death, as well as for protecting against cellular
stresses. An X-ray crystallographic study described
the formation of a pyramid shaped homotrimer that
is a proteolytically competent form of HtrA2;
however, little is known about effects of the trimeric
structure of HtrA2 on the natural substrates. In this
study, we generated the HtrA2 protein that has a
single point mutation at the homotrimerization motif
to assess relationship between structure and the
proteolytic activity of HtrA2 on its substrates. Using
gel filtration, a native gel electrophoresis system,
and a co-precipitation assay, we confirm that
phenylalanine 149 in HtrA2 is a crucial determinant
for the formation of the HtrA2 homotrimeric structure.
Moreover, we described that the HtrA2 monomeric
form abolished not only autoproteolytic activity, but
also the proteolytic activity against XIAP (X-linked
inhibitor of apoptosis protein) known as the HtrA2
substrate. Taken together, the results indicate that
the homotrimeric structure of HtrA2 is required for
executing its serine protease activity.
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참고문헌 (25)
참고문헌 신청
/ 1989 / and mapping of the Escherichia coli htrA gene whose product is essential for bacterial growth only at elevated temperatures : 1574 ~ 84
/ 1990 / essential for Escherichia coli survival at high temperatures is an endopeptidase : 1791 ~ 7
/ 1995 / Site-directed mutagenesis of the HtrA whose proteolytic activity is indispensable for Escherichia coli survival at elevated temperatures 163 : 47 ~ 52
/ 1999 / A temperature-dependent switch from chaperone to protease in a widely conserved heat shock protein 97 : 339 ~ 47
/ 2000 / Characterization of a novel human serine protease that has extensive homology to bacterial heat shock endoprotease HtrA and is regulated by kidney ischemia 275 : 258
/ 1990 / essential for Escherichia coli survival at high temperatures is an endopeptidase : 1791 ~ 7
/ 1995 / Site-directed mutagenesis of the HtrA whose proteolytic activity is indispensable for Escherichia coli survival at elevated temperatures 163 : 47 ~ 52
/ 1999 / A temperature-dependent switch from chaperone to protease in a widely conserved heat shock protein 97 : 339 ~ 47
/ 2000 / Characterization of a novel human serine protease that has extensive homology to bacterial heat shock endoprotease HtrA and is regulated by kidney ischemia 275 : 258
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2006 .08
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Purification and Characterization of Extracellular Temperature-Stable Serine Protease from Aeromonas hydrophila
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