지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.6
- 수록면
- 72 - 81 (10page)
이용수
초록· 키워드
오류제보하기
Canine distemper virus (CDV) infections cause high morbidity and mortality in dogs. Changes in the molecular biological characteristics of the Korean CDV strain over multiple cell passages have not been reported. We investigated the biological and genetic characteristics of CD1901-100 for use as an inactivated vaccine strain. Vero cells expressing the dog nectin-4 gene (Vero/dNectin-4 cells) were used to adapt CD1901, which was passaged 100 times in four types of cells. We assessed the cytopathic effects and used immunofluorescence assays to identify biological features of CD1901 and CD1901-100. Seven types of cells were used to explore the tropisms of the two CDV strains. The genetic analyses were based on whole-genome sequencing data. Vero cells expressing dog signaling lymphocyte activation molecule were infected with the two CDV strains and showed different cytopathic effects and fluorescence properties. CD1901-100 attained the highest viral titer of 10<SUP>6.5</SUP> TCID<SUB>50</SUB>/mL at 4 days post-inoculation; the overall highest virus titer of 10<SUP>7.0</SUP> TCID<SUB>50</SUB>/mL was that after growth in Vero/dNectin-4 cells. CD1901-100 exhibited 25 nucleotide mutations and 15 amino acid substitutions in six structural proteins compared to the CD1901 sequences. Of the six proteins, the F protein had the highest number of amino acid replacements (5/663, 0.75%). We constructed a Vero/dNectin-4 cell line and passaged CD1901 100 times in four types of cells. CD1901-100 propagated well in Vero/dNectin-4 cells. This will aid the development of an inactivated CDV vaccine.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Isolation and molecular characterizations of canine distemper virus from a naturally infected Korean dog using Vero cells expressing dog signaling lymphocyte activation molecule
Journal of Veterinary Science
2020 .09
-
Evaluation of Commercial Immunochromatographic Test Kits for the Detection of Canine Distemper Virus
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2020 .06
-
Suspension culture of Vero cells for the production of adenovirus type 5
Clinical and Experimental Vaccine Research
2020 .01
-
Canine regulatory T cells expressing CD4+and CD25+ are proliferated by interleukin-2 cytokine
한국실험동물학회 학술발표대회 논문집
2018 .01
-
Recharacterization of the Canine Adenovirus Type 1 Vaccine Strain based on the Biological and Molecular Properties
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2019 .09
-
T-Cells Modified with CD70 as an Alternative Cellular Vaccine for Antitumor Immunity
Cancer Research and Treatment
2020 .01
-
Extrinsic Acquisition of CD80 by Antigen-Specific CD8⁺ T Cells Regulates Their Recall Immune Responses to Acute Viral Infection
Immune Network
2019 .08
-
The Role of CD4 T Cell Help in CD8 T Cell Differentiation and Function During Chronic Infection and Cancer
Immune Network
2023 .10
-
CD5 Expression Dynamically Changes During the Differentiation of Human CD8+ T Cells Predicting Clinical Response to Immunotherapy
Immune Network
2023 .08
-
Characterization of pathogenicity and transmission ability of canine Influenza virus with partially truncated NS gene and application to vaccine candidate
한국실험동물학회 학술발표대회 논문집
2022 .01
-
Should the COVID-19 Vaccine be Administered Simultaneously with Other Vaccines?
Infection and Chemotherapy
2021 .09
-
Two Distinct Subsets Are Identified from the Peritoneal Myeloid Mononuclear Cells Expressing both CD11c and CD115
Immune Network
2019 .06
-
Exploring the experience of developing COVID-19 vaccines in Iran
Clinical and Experimental Vaccine Research
2023 .01
-
Establishment of Multiplex RT-PCR for Differentiating between the CD1901 and Lederle Strains of Canine Distemper Virus
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2022 .03
-
Review of COVID-19 Vaccines and Their Evidence in Older Adults
Annals of geriatric medicine and research
2021 .01
-
Therapeutic Vaccines and Nucleic Acid Drugs for Cardiovascular Disease
지질·동맥경화학회지
2024 .09
-
Vaccines in the United States: a systematic review on history of evolution, regulations, licensing, and future challenges
Clinical and Experimental Vaccine Research
2020 .01
-
Immunogenicity of a new, inactivated canine adenovirus type 2 vaccine for dogs
Clinical and Experimental Vaccine Research
2020 .01
-
Influenza Virus-Derived CD8 T Cell Epitopes: Implications for the Development of Universal Influenza Vaccines
Immune Network
2024 .06
-
Increased B Cell Understanding Puts Improved Vaccine Platforms Just Over the Horizon
Immune Network
2022 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2022-475-001505067