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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 92 - 103 (12page)
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초록· 키워드
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Objectives : The present study was examined to evaluate the anti-inflammatory effects of the Humulus japonicus MeOH extracts (HJE) in vivo.
Methods : The effects of HJE on anti-inflammation were measured by production of NO, iNOS (inducible Nitric Oxide Synthase), COX-2, IκBα (Inhibitor kappa B alpha), NFκB (Nuclear Factor kappa B), TNF-α
(Tumor Necrosis Factor-alpha) and IL-1β (Interleukin-1β), IL-6 in Raw 264.7 macrophage cells stimulated with LPS.
Results : 1. All concentrations of HJE(0.03 and 0.10 ㎎/㎖) had no significant cytotoxicity in Raw 264.7 cell during the entire experimental period.
2. The level of NO and iNOS in culture medium was dramatically increased by LPS application. However, these increases were dose-dependently(0.03 and 0.10 ㎎/㎖) attenuated by treatment with HJE.
3. HJE extract reduced PGE2 levels in a dose-dependent manner as a consequence of inhibition of COX-2 protein expression in Raw 264.7 macrophage cells stimulated with LPS.
4. 0.10 ㎎/㎖ HJE significantly inhibited the phosphorylation of IκBα indicating the suppression of NF-κB pathway in Raw 264.7 macrophage cells stimulated with LPS.
5. 0.10 ㎎/㎖ HJE significantly inhibited the production of TNF-α in Raw 264.7 macrophage cells stimulated with LPS.
6. All concentrations of HJE significantly inhibited the production of IL-1β, IL-6 in Raw 264.7 macrophage cells stimulated with LPS.
Conclusions : These results provide evidences that therapeutic effect of HJE on heat syndrome, especially due to the acute inflammation, are partly due to the reduction of some of inflammatory factors by inhibiting iNOS and COX-2 through the suppression of p-IκBα. Moreover, it suggests that the mechanism
of action of HJE comes from the suppression of inflammatory mediators, such as NO, PGE2 and pro-inflammatory cytokines.
목차
등록된 정보가 없습니다.
참고문헌 (31)
참고문헌 신청
Chun SC / 2007 / Extract of Moutan Cortex in LPS-Activated Raw 264.7 cells / Evid Based Complement Alternat Med 4 (3) : 327 ~ 333
Grosch S / 2006 / Cyclooxygenase-2 (COX-2)- independent anticarcinogenic effects of selective COX-2 inhibitors / J Natl Cancer Inst 98 (11) : 736 ~ 747
Higuchi M / 1990 / Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanism acts as synergistically as the major cytolytic mech
Kawamata H / 2000 / Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated Raw 264.7 cells, a murine macrophage cell line / Am J Chin Med 2
Lee AK / 2003 / Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-α and COX-2 expression by sauchinone effects on I-κB α phosphorylation, C/EBP and AP
Grosch S / 2006 / Cyclooxygenase-2 (COX-2)- independent anticarcinogenic effects of selective COX-2 inhibitors / J Natl Cancer Inst 98 (11) : 736 ~ 747
Higuchi M / 1990 / Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanism acts as synergistically as the major cytolytic mech
Kawamata H / 2000 / Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated Raw 264.7 cells, a murine macrophage cell line / Am J Chin Med 2
Lee AK / 2003 / Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-α and COX-2 expression by sauchinone effects on I-κB α phosphorylation, C/EBP and AP
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