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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 1,092 - 1,100 (9page)
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Purpose: Long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) has been implicated as an oncogenein the development and progression of osteosarcoma. This study aims to explore the mechanism of NEAT1 in osteosarcoma.
Materials and Methods: Expressions of NEAT1 and miR-194 in osteosarcoma tissues and cells were detected by quantitative real-time PCR. The effects of NEAT1 knockdown or miR-194 overexpression on cell proliferation, invasion, and apoptosis were determinedby 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT) assay, transwell invasive assay, and flow cytometry analysis, respectively. Luciferase reporter assay was performed to observe the possible interaction between NEAT1 and miR-194.
Results: NEAT1 was upregulated and miR-194 was downregulated in osteosarcoma tissues and cells. Knockdown of NEAT1 or overexpression of miR-194 suppressed proliferation and invasion and induced apoptosis of osteosarcoma cells in vitro. Luciferase reporter assay validated that NEAT1 could interact with miR-194 and negatively modulated its expression. Furthermore, inhibitionof miR-194 reversed the suppression of proliferation and invasion and the promotion of apoptosis induced by NEAT1 depletionin osteosarcoma cells.
Conclusion: Knockdown of NEAT1 suppressed proliferation and invasion and induced apoptosis in osteosarcoma cells by inhibitingmiR-194 expression.
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참고문헌 (25)
참고문헌 신청
Cheetham SW / 2013 / Long noncoding RNAs and the genetics of cancer / Br J Cancer 108 : 2419 ~ 2425
Chen L / 2012 / miR-137 is frequently down-regulated in glioblastoma and is a negative regulator of Cox-2 / Eur J Cancer 48 : 3104 ~ 3111
Choudhry H / 2015 / Tumor hypoxia induces nuclear paraspeckle formation through HIF-2α dependent transcriptional activation of NEAT1leading to cancer cell survival / Oncoge
Cong M / 2016 / Long non-coding RNA tumor suppressor candidate 7 functions as a tumor suppressor and inhibits proliferation in osteosarcoma / Tumour Biol 37 : 9441 ~ 9450
Dong P / 2011 / MicroRNA-194 inhibits epithelial to mesenchymal transition of endometrial cancer cells by targeting oncogene BMI-1 / Mol Cancer 10 : 99 ~
Chen L / 2012 / miR-137 is frequently down-regulated in glioblastoma and is a negative regulator of Cox-2 / Eur J Cancer 48 : 3104 ~ 3111
Choudhry H / 2015 / Tumor hypoxia induces nuclear paraspeckle formation through HIF-2α dependent transcriptional activation of NEAT1leading to cancer cell survival / Oncoge
Cong M / 2016 / Long non-coding RNA tumor suppressor candidate 7 functions as a tumor suppressor and inhibits proliferation in osteosarcoma / Tumour Biol 37 : 9441 ~ 9450
Dong P / 2011 / MicroRNA-194 inhibits epithelial to mesenchymal transition of endometrial cancer cells by targeting oncogene BMI-1 / Mol Cancer 10 : 99 ~
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