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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 336 - 345 (10page)
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초록· 키워드
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Purpose: Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) has been deemed an oncogene in many humancancers. However, the underlying mechanism of NEAT1 in nasopharyngeal carcinoma (NPC) progression remains largelyunclear. Materials and Methods: Quantitative real-time PCR assay was performed to assess the expression of NEAT1 and miR-34a-5p inNPC tissues and cells. Western blot analysis was used to observe cell epithelial to mesenchymal transition (EMT) and the activationof Wnt/β-catenin signaling in 5-8F cells. MiRNA directly interacting with NEAT1 were verified by dual-luciferase reporter assayand RNA immunoprecipitation. Cell proliferation ability was determined by CCK-8 assay, and cell migration and invasion capacitieswere assessed by transwell assays. An animal model was used to investigate the regulatory effect of NEAT1 on tumorgrowth in vivo. Results: Our data revealed that NEAT1 is upregulated, while miR-34a-5p is downregulated in NPC tissues and cell lines. NEAT1knockdown repressed tumor growth in vitro and in vivo. Additionally, we discovered that NEAT1 directly binds to miR-34a-5pand suppresses miR-34a-5p expression. Moreover, NEAT1 knockdown exerted suppression effects on cell proliferation, migration,invasion, and EMT by miR-34a-5p. NEAT1 knockdown blocked Wnt/β-catenin signaling via miR-34a-5p. Conclusion: Our study demonstrated that NEAT1 targets miR-34a-5p at least partly to drive NPC progression by regulating Wnt/β-catenin signaling, suggesting a potential therapeutic target for NPC.
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참고문헌 (36)
참고문헌 신청
Lee VH / 2018 / Management of nasopharyngeal carcinoma : is adjuvant therapy needed / J Oncol Pract 14:594~602
Yip TT / 2014 / Application of circulating plasma/serum EBV DNA in the clinical management of nasopharyngeal carcinoma / Oral Oncol 50:527~538
Wu F / 2014 / Concurrent chemoradiotherapy in locoregionally advanced nasopharyngeal carcinoma : treatment outcomes of a prospective, multicentric clinical study / Radiother Oncol 112:106~111
Lai SZ / 2011 / How does intensity-modulated radiotherapy versus conventional two-dimensional radiotherapy influence the treatment results in nasopharyngeal carcinoma patients? / Int J Radiat Oncol Biol Phys 80:661~668
Spizzo R / 2012 / Long non-coding RNAs and cancer : a new frontier of translational research / Oncogene 31:4577~4587
Yip TT / 2014 / Application of circulating plasma/serum EBV DNA in the clinical management of nasopharyngeal carcinoma / Oral Oncol 50:527~538
Wu F / 2014 / Concurrent chemoradiotherapy in locoregionally advanced nasopharyngeal carcinoma : treatment outcomes of a prospective, multicentric clinical study / Radiother Oncol 112:106~111
Lai SZ / 2011 / How does intensity-modulated radiotherapy versus conventional two-dimensional radiotherapy influence the treatment results in nasopharyngeal carcinoma patients? / Int J Radiat Oncol Biol Phys 80:661~668
Spizzo R / 2012 / Long non-coding RNAs and cancer : a new frontier of translational research / Oncogene 31:4577~4587
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