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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 243 - 250 (8page)
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초록· 키워드
오류제보하기
A bone marrow biopsy that has undergone decalcification with 10% nitric acid could not be used for various pathological tests and had extremely limited reproducibility. The fixing solution of each experimental group was differentiated in usage, one solution including acid and the other not. The use of the decalcification solution was separated into either acidic or alkaline (EDTA), and further experiments were conducted with differing time phases. When using a fixing solution and decalcification solution which included acid, the specimens were faulty to the extent that all pathological tests were impossible. However specimens that were processed with an EDTA type decalcification solution did not display a non-specific reaction in EBV ISH and were even able to produce results that were at a level suited to various studies or a pathological diagnosis in the FISH, DNA, RNA tests. By improving the fixing and decalcification of bone marrow biopsy, the study was able to make possible ISH, FISH, DNA tests as well as RNA study, and secured the sensitivity, specificity, and reproducibility of various test methods. The stabilization of various test methods that use bone marrow biopsy contributes to the diagnosis, prognosis, prediction, treatment of the patient and provide guidelines for decision-making.
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등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
Alers JC / 1999 / Effect of bone decalcification procedures on DNA in situ hybridization and comparative genomic hybridization: EDTA is highly preferable to a routinely used
Brown RS / 2002 / Dogan A. Routine acid decalcification of bone marrow samples can preserve DNA for FISH and CGH studies in metastatic prostate cancer / J Histochem Cytochem
Callis G / 1998 / Decalcification of bone: Literature review and practical study of various decalcifying agents, methods, and their effects on bone histology / J Histotechnol
Castania VA / 2015 / Advantages of a combined method of decalcification compared to EDTA / Microsc Res Tech 78 (2) : 111 ~ 118
Choi YI. / 1992 / Influence of Decalcification on the Immunoreactivity of Paraffin Sections. / Korean Journal of Medical Technologists 24 : 90 ~ 100
Brown RS / 2002 / Dogan A. Routine acid decalcification of bone marrow samples can preserve DNA for FISH and CGH studies in metastatic prostate cancer / J Histochem Cytochem
Callis G / 1998 / Decalcification of bone: Literature review and practical study of various decalcifying agents, methods, and their effects on bone histology / J Histotechnol
Castania VA / 2015 / Advantages of a combined method of decalcification compared to EDTA / Microsc Res Tech 78 (2) : 111 ~ 118
Choi YI. / 1992 / Influence of Decalcification on the Immunoreactivity of Paraffin Sections. / Korean Journal of Medical Technologists 24 : 90 ~ 100
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