고양이 전농화 실험을 통한 Ethacrynic Acid와 체중 간의 상관성 고찰

장정훈; 이호선; 김혜영; 이준호; 장선오; 오승하; 박민현

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자료유형: 학술저널

저자정보: 장정훈 이호선 김혜영 이준호 장선오 오승하 박민현

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제53권 제12호

발행연도: 2010.1

수록면: 749 - 754 (6page)

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Background and Objectives Co-administration of kanamycin (KM) with the loop diuretic ethacrynic acid (EA) has been known to produce a rapid and profound hearing loss in adult animals. The objective of this study was to see if monitoring the hearing status during intravenous infusion of EA could minimalize individual variability and to evaluate the correlation between the dose of EA and the body weight (wt). Materials and Method Twenty cats with the mean age of 24 weeks±3.7 (range, 20.6-28.3 weeks) and the mean weight of 3.27 kg±0.75 (range 2.4-4.75 kg) received a subcutaneous injection of KM (300 mg/kg) followed by an intravenous infusion of EA (1 mg/min). Click evoked auditory brainstem responses (ABRs) were recorded to monitor the hearing during the infusion. When the ABR thresholds rose to levels in excess of 90 dB SPL, the infusion of EA was stopped. The histopathologies for sections of apex, middle, base of cochlea were examined after 6 months. Results There was a significant positive correlation (p<.001, r2=.583) between the EA dose and body weight. Cochlear histopathology showed an absence of organ of Corti and decrease of spiral ganglion cells in the majority of cochleas, especially in the basal turn. The extent of loss of spiral ganglion cells was dependent on their distance from the round window. Conclusion Monitoring the animal’s hearing status during the procedure ensured that the dose of EA was optimized for individual animals. Thus, the positive correlation between the EA dose and body weight should be considered should in designing the animal models of controlled high frequency hearing loss. Korean J Otorhinolaryngol-Head Neck Surg 2010;53:749-54

#Ethacrynic acid ㆍCochlea ㆍDeafness ㆍAminoglycosides.

참고문헌 (20)

참고문헌 신청

Begg EJ / 1995 / Aminoglycosides--50 years on / Br J Clin Pharmacol 39 (6) : 597 ~ 603

Bowes T / 2008 / Novel mitochondrial extensions provide evidence for a link between microtubule-directed movement and mito chondrial fission / Biochem Biophys Res Commun 376

Brugge JF / 1978 / Signs of functional maturation of peripheral auditory system in discharge patterns of neurons in anteroventral cochlear nucleus of kitten / J Neurophysiol

Brummett RE / 1981 / Comparative ototoxicity of bumetanide and furosemide when used in combination with kanamycin / J Clin Pharmacol 21 (12) : 628 ~ 636

Cody AR / 1983 / Variability of noise-induced damage in the guinea pig cochlea: electrophysiological and morphological correlates after strictly controlled exposures / Hear R

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