두경부 편평상피세포암 세포주 PCI-1, PCI-13과 PCI-50의 염색체이상 분석

문영은; 최영석; 연제엽; 지아람; 이동욱

추천

검색

자료유형: 학술저널

저자정보: 문영은 최영석 연제엽 지아람 이동욱

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제52권 제9호

발행연도: 2009.1

수록면: 741 - 750 (10page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background and Objectives The knowledge about chromosomal aberrations manifestated in cancer has been spotlighted recently. The genetic analyses based on the knowledge about chromosomal aberrations are important for the development of diagnotic methods and evaluation of prognostic factors in cancers. Comparative genomic hybridization (CGH) is a powerful tool for evaluating chromosomal aberrations and array CGH significantly enhances these diagnostic effectiveness. The incidence of head and neck squamous cell carcinoma (HNSCC) has been increasing worldwide but the treatment outcomes still have been limited. The aim of this study is to evaluate the location of chromosomal aberrations in HNSCC cell lines with the combination of CGH and array CGH. Materials and Method The locations of chromosomal aberrations were evaluated in 3 HNSCC cell lines (PCI-1, PCI-13, PCI-50) using the combination of CGH and array CGH. Results The sites of chromosomal gain shown by CGH in all 3 cell lines were 8q22-qter, 9q32- qter, 10q22, 10q26-qter, 16q12.1-qter, 17p10-p13, 17q21-qter, 19p13.2-pter and 20q. The chromsomal loss found in 2 cell lines were 3p, 4q21-qter, and 18q21-qter. In array-CGH, gained loci were AHRR, MYT1 and PTGIS, etc. Loci of genetic losses were ELAVL4 and GRM7. Conclusion In this study, we identified various genetic gains and losses using CGH and high resolution array-CGH. These data about the patterns of chromosomal aberrations in HNSCC cell lines would be a basic step for understanding more detailed genetic events in the carcinogenesis. CGH combined with array CGH can be a powerful option for transitional oncologic research.

#Squamous cell carcinoma·Head and neck cancer·Nucleic acid hybridization· Chromosome

참고문헌 (20)

참고문헌 신청

Agochiya M / 1999 / Increases dosage and amplification of the focal adhesion kinase gene in human cancer cells / Oncogene 18 (41) : 5646 ~ 5653

Ashman JN / 2003 / Prognostic value of genomic alterations in head and neck squamous cell carcinoma detected by comparative genomic hybridisation / Br J Cancer 89 (5) : 864

Bockmuhl U / 1998 / Genomic alterations associated with malignancy in head and neck cancer / Head Neck 20 (20) : 145 ~ 151

Frigola J / 2005 / Hypermethylation of the prostacyclin synthase (PTGIS) promoter is a frequent event in colorectal cancer and associated with aneuploidy / Oncogene 24 (49)

Gollin SM / 2001 / Chromosomal alterations in squamous cell carcinomas of the head and neck: window to the biology of the disease / Head Neck 23 (3) : 238 ~ 253

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!