두경부 편평세포암종에서 메틸화 양상과 이형 접합성 소실

홍명화; 홍승진; 유문간; 선동일; 조승호; 김민식

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자료유형: 학술저널

저자정보: 홍명화 홍승진 유문간 선동일 조승호 김민식

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제50권 제2호

발행연도: 2007.1

수록면: 145 - 156 (12page)

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Background and Objectives：Subjects and Method：Both normal mucosa and tumor tissue samples were secured from 17 cases to a total of 34 samples to be examined with a methylation- specific PCR (LOH) using a panel of 41 microsatellite markers on 8 chromosomes. Results：The patern of methylation changes between the paired normal mucosa and tumor site was variable. Of the total of 206 cases examined for the methylation status of non-CpG island, 34 cases showed hy-pomethylation changes, 26 cases hypermethylation changes, and 31 cases no methylation changes. Regions containting CpG is-lands had 8 cases showing hypomethylation changes, 17 cases hypermethylation changes, and 31 cases of no methylation ch-downstream 0.7 kbp, p16 upstream 1.0 kbp, and hMLH1 upstream 1.0 kbp showed hypomethylation, whereas BGLAP upstream 4.5 kbp, Runx3 upstream 1.7 kbp, KIA downstream 0.4 kbp showed hypermethylation. However, the rest of the genes were not changed. In 29 tumor foci, a LOH was found most frequently on the chromosomes 3p, 8p, 9p, and 13q. Interestingly, alth-ough other previous reports have not reported the detection of 8p chromosomal loss in head and neck cancer, this study frequ-ently detected 8p chromosomal loss. Chromosomal loss yielded an overall mean value of 4.79± 2.2 per tumor focus. A special relationship could not be drawn based on thand hMLH1, there were diferences betwen the hypomethylation. Genetic instability was raised when hypomethylation incre-ased. Conclusion：This study showed that the head and neck cancer and its progresion generally need the proper level of chro-mosomal losses to accomplish cancer progression or development. Methylation pattern and LOH might be important rules and target event in head and neck cancer. In the future, experiments to find the point of genetic modification will help the way to pre-vent the cancer. (Korean J Otolaryngol 2007 ;50 :145-56)

#단측성 염색체소실·메틸화·두경부암. #Los of heterozygosity· Methylation· Head and neck cancer.

참고문헌 (20)

참고문헌 신청

/ 1992 / THe essentials of DNA methylation : 4231 ~ 6

/ 1994 / An allelotype of squamous carcinoma of the head and neck using microsatellite markers

/ 1996 / Me-thylation-specific PCR: A novel PCR assay for methylation status of CpG island 93 (18) : 9821 ~ 9826

/ 1998 / Alterations in DNA methylation afundamental aspect of meolpasia 72 : 141 ~ 196

/ 1999 / Are there several types of coloretal car-cinimas? corelations with genetic data 8 : S13 ~ S20

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