Quantitative Analysis of Cancer-associated Gene  Methylation Connected to Risk Factors in Korean  Colorectal Cancer Patients

강호진; 김은정; 김병권; 유창훈; 이상용; 김동일; 홍영습

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자료유형: 학술저널

저자정보: 강호진 김은정 김병권 유창훈 이상용 김동일 홍영습

저널정보: 대한예방의학회 예방의학회지 예방의학회지 제45권 제4호

발행연도: 2012.1

수록면: 251 - 258 (8page)

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Objectives: The purpose of this paper was to elucidate the potential methylation levels of adjacent normal and cancer tissues by comparing them with normal colorectal tissues, and to describe the correlations between the methylation and clinical parameters in Korean colorectal cancer (CRC) patients. Methods: Hypermethylation profiles of nine genes (RASSF1, APC, p16INK4a, Twist1, E-cadherin, TIMP3, Smad4, COX2, and ABCB1) were examined with 100 sets of cancer tissues and 14 normal colorectal tissues. We determined the hypermethylation at a given level by a percent of methylation ratio value of 10 using quantitative methylation real-time polymerase chain reaction. Results: Nine genes’ hypermethylation levels in Korean CRC patient tissues were increased more higher than normal colorectal tissues. However, the amounts of p16INK4a and E-cadherin gene hypermethylation in normal and CRC tissues were not significantly different nor did TIMP3 gene hypermethylation in adjacent normal and cancer tissues differ significantly. The hypermethylation of TIMP3, Ecadherin,ABCB1, and COX2 genes among other genes were abundantly found in normal colorectal tissues. The hypermethylation of nine genes’ methylation in cancer tissues was not significantly associated with any clinical parameters. In Cohen’s kappa test, it was moderately observed that RASSF1 was related with E-cadherin, and Smad4 with ABCB1 and COX2. Conclusions: This study provides evidence for different hypermethylation patterns of cancer-associated genes in normal and CRC tissues,which may serve as useful information on CRC cancer progression.

#Hypermethylation #Colorectal neoplasms #Genetic markers

참고문헌 (25)

참고문헌 신청

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