ZnSO₄비내 점적으로 손상된 마우스 후각상피 및후구의 회복경과에 따른 면역조직학적 연구

김명원; 박용진; 문영화; 김병국; 곽봉기

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자료유형: 학술저널

저자정보: 김명원 박용진 문영화 김병국 곽봉기

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제49권 제4호

발행연도: 2006.1

수록면: 395 - 401 (7page)

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Background and Objectives：The purpose of this study was to evaluate the time course of functional and anatomical recovery of the olfactory epithelium (OE) and olfactory bulb (OB) following intranasal instillation of 1% ZnSO4 in mice. Materials and Method：Two groups of mice, normal control group (intranasal instillation of normal saline, n=6) and experimental group (intranasal instillation of ZnSO4, n=18), were studied. Tissues of olfactory pathways were obtained at 1, 3 and 5 weeks after bilateral intranasal instillation of 1%, 100 μl ZnSO4 solution, and processed for immunohistochemistry using antisera, olfactory marker protein (OMP), proliferating cell nuclear antigen (PCNA) and tyrosine hydroxylase (TH) to evaluate the olfactory regeneration. For histological study, OE thickness stained with hematoxylin-eosin was analyzed. Results：At 1 week after ZnSO4 intranasal instillation, the lowest peak of OMP expression in OE appeared. Then the number of OMP-positive cells increased progressively at weeks. However, PCNA expression in OE showed quite the opposite. In the corresponding OB at 1 week, there was decrease of TH-positive cells and at 3 weeks, there was few TH-positive cells. At 5 weeks, there was increase in the number of TH-positive cells again. OE thickness was reduced to 20% of control OE at 1 week, and was significantly recovered to 80% of control OE at 5 weeks. Conclusion：Our results demonstrated that intranasal instillation of 1% ZnSO4 to mice produces a brief, reversible but essentially complete destruction of functional connection from the olfactory epithelium to the main olfactory bulb. (Korean J Otolaryngol 2006;49:395-401)

#ZnSO4·마우스·후각계·면역조직화학법. #ZnSO4·Mice·Olfactory pathways·Immunohistochemistry.

참고문헌 (14)

참고문헌 신청

/ / Chemical deafferentation of the olfactory bulb Plasticity ofthe levels of tyrosine hydroxylase gran (t-reisfeld n repinephrine) : 365 ~ 77

/ / Denervation of the primaryolfactory pathway in mice Long-term effect of intranasal ZnSO4irrigation on behavior : 271 ~ 85

/ 1983 / Transneuronal regulationof tyrosine hydroxylase expression in olfactory bulb of mouse and rat 3 : 69 ~ 78

/ 1984 / OlfactoryBulb dopamine neurons survive deafferentation-induced loss oftyrosine hydroxylase 11 : 605 ~ 15

/ 1984 / Replication and differentiation of olfactoryreceptor neurons following axonotomy in the adult hamster:A morphologicalanalysis of postnatal neurogenesis 225 : 201 ~

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