간세포성장인자가 하인두 편평세포암 세포주의 증식, 분산과 이동에 미치는 영향

배준호; 최은창; 문성균; 이재호; 김철호

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자료유형: 학술저널

저자정보: 배준호 최은창 문성균 이재호 김철호

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제48권 제2호

발행연도: 2005.1

수록면: 208 - 215 (8page)

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Background and Objectives：Hepatocyte growth factor (HGF) is known to stimulate motility, invasivenes, proliferation, and morphogenesis of endothelial cells. Recent reports revealed that this growth factor is also related to tumor invasion and metas-tasis. We examined the role of HGF/c-Met on the proliferation, dispersion and migration of FaDu cell, a hypopharyngeal squa-mous cell carcinoma cell line. Materials and Method：We performed RT-PCR and Western blot in FaDu. Proliferation of the FaDu cels was assayed by counting the number of cells after treatment by HGF of diferent concentrations of 0, 10, 30 ng/mL. Dispersion of the cells was observed by measuring the separation and morphologic changes of cells after the colony of FaDu cells was formed in the media and then treated with HGF of 10 ng/mL or 30 ng/mL for 24 hours. Tumor cel migration was assesed by wound healing asay. Lastly, we examined the enhancement of HGF production in human fibroblast (MRC-5) by putative inducer secreted from FaDu cels. Results：popharyngeal cel line while that of HGF was not. Exogenous HGF significantly enhanced the growth of FaDu in a dose-dependent manner 30 ng/ml (p<0.05). HGF stimulated the dispersion and enhanced the migration of cancer cells in a dose-dependent manner (p<0.05). HGF produced by human stromal fibroblast (MRC-5) was increased by a certain inducer ori-ginated from FaDu cells (p<0.05). Conclusion：These results sugest that HGF may play an important role in the progresion of hypopharyngeal cancer through the enhancement of proliferation, dispersion and migration.

#간세포성장인자·c-Met·하인두암·증식·전이. #Hepatocyte growth factor· c-Met· Hypopharyngeal cancer· Proliferation· Migration.

참고문헌 (23)

참고문헌 신청

/ / Induction of hepatocyte growth factor in fibroblasts by tumor-derived factors affects invasive growth of tumor cells In vitro analysis of tumor-stromal interactions ta (

/ 1984 / Partial purification and characterization of hepatocyte growth factor from serum of hepatectomized rats 122 : 1450 ~ 9

/ 1989 / A study of an in vitro model for invasion of oral squamous cell carcinoma 18 : 498 ~ 501

/ 1990 / Scatter factor:Molecular characteristics and effect on the invasiveness of epithelial cells 111 : 2097 ~ 108

/ 1992 / Tumour invasion:Effects of cell adhesion and motility 2 : 163 ~ 9

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