간세포성장인자가 하인두 편평세포암 세포주에서 MatrixMetalloproteinase(MMP)-2, 9과 Urokinase TypePlasmogen Activator에 미치는 영향

배준호; 최은창; 김철호; 문성균; 이재호

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자료유형: 학술저널

저자정보: 배준호 최은창 김철호 문성균 이재호

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제48권 제6호

발행연도: 2005.1

수록면: 788 - 795 (8page)

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Background and Objectives：Recent reports revealed that hepatocyte growth factor (HGF) is related to tumor invasion andmetastasis. Activation of the urokinase-type plasminogen activator (u-PA)/plasmin proteolytic network and matrix metalloproteinasehas been shown to play a key role in tumor invasion and dissemination of various malignancies. So we examined theeffect of HGF/c-Met on MMP-2, 9 and u-PA in FaDu cell, a hypopharyngeal squamous cell carcinoma cell line. Materials andMethod：We performed RT-PCR and Western blot in FaDu. Tumor cell invasiveness was assessed by the membrane invasionassay (using Transwell chamber). To examine the role of MMP-2, 9 and the relation between HGF and MMP in the invasion ofhypopharyngeal cancer, RT-PCR and zymography were performed in FaDu cells. We tested to confirm the HGF-mediatedplasmin activation. Results：The expressions of c-Met mRNA and protein were detected in the hypopharyngeal cell line whilethat of HGF was not. HGF markedly enhanced the invasion of cancer cells in a Transwell invasion chamber in a dose-dependentmanner (p<0.05). The expression of MMP was detected in hypopharyngeal cancer cells and exogenous HGF slightly enhancedthe induction of MMP-2 activity in zymogram analysis. The activity of u-PA was detected in FaDu and HGF (above 10units/mL) enhanced the activity of u-PA (p<0.05). Conclusion：These results suggest that HGF may play an important role inhypopharyngeal cancer through the activation of u-PA and matrix metalloproteinase.

#간세포성장인자·c-Met·하인두암·침습·urokinase-type plasminogen activator·matrix metalloproteinase. #Hepatocyte growth factor·c-Met #Hypopharyngeal cancer·Invasion·Urokinase-type plasminogen activator·Matrix metalloproteinase.

참고문헌 (21)

참고문헌 신청

/ / Effect of HGF inproliferation dispersion and migration of hypopharyngeal squamouscell carcinoma

/ / Effect of plasminogen activator andthrombin on glycoprotein and collagenous components of basementmembrane : 4629 ~ 36

/ / Molecular characteristics and effect on the invasiveness ofepithelial cells : 2097 ~ 108

/ / Theprognostic relevance of plasminogen activators uPA and tPA inhuman breast cancer : 695 ~ 702

/ / Type IV collagenases in invasivetumors : 209 ~ 18

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