지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.9
- 수록면
- 117 - 123 (7page)
이용수
초록· 키워드
오류제보하기
This study was designed to investigate the role of the hepatocyte growth factor (HGF) with regards to differentiation of somatic stem cells originating from the human umbilical cord blood (UCB) into hepatic lineage cells in vitro culture system. Methods: Mononuclear cells from UCB were cultured with and without HGF based on the fibroblast growth factor (FGF)-1, FGF-2, and stem cell factor. The cultured cells were confirmed by immunofluorescent staining analysis with albumin (ALB), cytokeratin-19 (CK-19), and proliferating cell nuclear antigen (PCNA) MoAb. ALB and CK-18 mRNA were also evaluated by reverse transcription-polymerase chain reaction. In order to observe changes in proliferating capacity with respect to the cultured period, CFSE with affinity to proliferating cells were tagged and later underwent flow cytometry. Results: In the HGF-treated group, cultured cells had a large oval shaped appearance with adherent, but easily detachable characteristics. In the HGF-non treated group, these cells were spindle-shaped with strong adherent characteristics. Expressions of ALB and CK-19 were evident in HGF-treated group compared to non-expression of those in to HGF-non treated group. Dual immunostaining analysis of the ALB producing cells showed presence of PCNA in their nuclei, and ALB and CK-18 mRNA were detected on the 21st day of cultured cells in the HGF-treated group. Conclusion: Our findings suggest that HGF has a pivotal role in differentiating somatic stem cells of human UCB into hepatic lineage cells in vitro.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
간세포성장인자가 하인두 편평세포암 세포주의 증식, 분산과 이동에 미치는 영향
대한이비인후-두경부외과학회지
2005 .01
-
하인두 편평세포암주의 증식과 침습을 유도하는 간세포성장인자에 대한 녹차추출물 억제효과
대한이비인후-두경부외과학회지
2008 .01
-
The differentiation of human multipotent adult progenitor cells into hepatocyte-like cells induced by coculture with human hepatocyte line L02
Annals of Surgical Treatment and Research
2014 .12
-
Expression of Hepatocyte Growth Factor/c-met by RT-PCR in Meningiomas
Journal of Pathology and Translational Medicine
2011 .01
-
간세포성장인자가 하인두 편평세포암 세포주에서 MatrixMetalloproteinase(MMP)-2, 9과 Urokinase TypePlasmogen Activator에 미치는 영향
대한이비인후-두경부외과학회지
2005 .01
-
In vitro Differentiation of Human Umbilical Cord-derived Stem Cells and Human Amnion-derived Stem Cells into Hepatocyte-like Cells
대한생식의학회 학술대회
2007 .01
-
제대혈 내피기원세포 및 간엽줄기세포의 분화에 대한 연구
대한구강악안면외과학회지
2005 .01
-
Function of hepatocyte growth factor in gastric cancer proliferation and invasion
Journal of Yeungnam Medical Science
2020 .01
-
Neuron-Like Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells
Yonsei Medical Journal
2011 .01
-
Role of HGF/c-Met in Serum-Starved ARPE-19 Cells
Korean Journal of Ophthalmology
2007 .01
-
시험관내 배양된 제대혈 모세포에서의 신경항원 발현
대한신경외과학회지
2001 .01
-
Invasion-Metastasis by Hepatocyte Growth Factor/c-Met Signaling Concomitant with Induction of Urokinase Plasminogen Activator in Human Pancreatic Cancer: Role as Therapeutic Target
Cancer Research and Treatment
2003 .01
-
유방암에서 혈중 간세포 성장인자(Hepatocyte Growth Factor)측정의 임상적 유용적 평가
Annals of Laboratory Medicine
2003 .01
-
하인두 편평세포암에서 간세포성장인자와 그 수용체인 c-Met의 발현
대한이비인후-두경부외과학회지
2004 .01
-
Guided Differentiation of Human Embryonic Stem Cells into Hepatocytes;From Endoderm Specification to Hepatic Maturation
대한생식의학회 학술대회
2006 .01
-
줄기세포 치료의 최신 지견
Obstetrics & Gynecology Science
2005 .01
-
Retinoic Acid Increases the Cell Cycle Progression of Human Gingival Fibroblasts by Increasing Cyclin E and CDK 2 Expression and Decreasing $p21^{WAF1/CIP1}$ and $p16^{INK4A}$ Expression
International Journal of Oral Biology
2012 .01
-
Hepatic differentiation from human embryonic stem cells using stromal cells
대한외과학회 학술대회 초록집
2010 .11
이 논문의 저자 정보
최근 본 자료
댓글(0)
0