소아 삼출성 중이염에서 중합효소연쇄반응(PCR)을 이용한  진균 및 세균의 검출과 관련된 임상 인자의 분석

이재은; 전경명; 이진우; 이종길; 장철훈

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자료유형: 학술저널

저자정보: 이재은 전경명 이진우 이종길 장철훈

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제51권 제11호

발행연도: 2008.1

수록면: 960 - 966 (7page)

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Several hypotheses have been proposed in the etiopathogenesis of otitis media with effusion (OME). The bacterial or fungal infection may also play a major role in the pathogenesis of OME. To investigate the relationship between bacteria and fungi as pathogens in OME and to analyze the incidence, the authors evaluated the pathogens of OME using polymerase chain reaction (PCR) technique, which is known to be more sensitive and specific than conventional bacterial and fungal culture. Subjects and Method：Twenty-three children (thirty ears), who were diagnosed with OME and had underwent ventilation tube insertion, were evaluated in the department of ORL-HNS, University Hospital, from May 2006 to March 2007. The middle ear effusion, obtained during the procedure, was evaluated for the identification of bacteria and fungi by PCR. Results：Among 30 ears, viable pathogenic bacteria were detected in 20 ears (66.6%) and fungi in 13 ears (43.3%). The bacterial pathogens included Haemophilus influenzae (13 ears), followed by Streptococcus pneumonize (6 ears). The fungi detected from PCR were Candida albicans (4 ears-30.8%), Aspergillus niger (2 ears-15.4%) and Paecilomyces lilacinus (2 ears-15.4%). Additional pathogens include Basidiomycete yeast, Saccharamycete sp., Eurotium rubrum, Dothioraceae sp. and Stemphylium solani. Detection of fungal DNA was more common in patients with cleft palate and in recurrent cases with statistical significance. Conclusion： The use of PCR of middle ear effusion is effective for the detection of pathogens in patients with OME. While bacteria were thought to be the causative pathogen, this study suggests the etiological role of fungi in the pathogenesis of OME. However, the relationship between fungi and OME requires further studies. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:960-6)

#Otitis media with effusion #OME)·Fungi·Bacteria·Polymerase chain reaction #PCR).

참고문헌 (22)

참고문헌 신청

Aul JJ / 1998 / Comparative evaluation of culture and PCR for the detection and determination of persistence of bacterial strains and DNAs in the Chinchilla laniger model of

Brook I / 2000 / Aerobic and anaerobic bacteriology of concurrent chronic otitis media with effusion and chronic sinusitis in children / Arch Otolaryngol Head Neck Surg 126

Chen YC / 2000 / Identification of medically important yeasts using PCR-based detection of DNA sequence polymorphisms in the internal transcribed spacer 2 region of the rRNA

Dingman JR / 1998 / Correlation between presence of viable bacteria and presence of endotoxin in middle-ear effusions / J Clin Microbiol 36 (11) : 3417 ~ 3419

Gok U / 2001 / Bacteriological and PCR analysis of clinical material aspirated from otitis media with effusions / Int J Pediatr Otorhinolaryngol 60 (1) : 49 ~ 54

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