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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 90 - 97 (8page)
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The cytoskeletal β-actin gene and its 5'-upstream region were isolated and characterized in the rockbream (Oplegnathus fasciatus). Complementary DNA of the rockbream β-actin represented a 1,125 bp of an open reading frame encoding 375 amino acids, and the rockbream β-actin cDNA and deduced amino acid sequences were highly homologous to those of other vertebrate orthologs. At the genomic level, the β-actin gene also exhibited an organization typical of vertebrate cytoskeletal actin genes (2,159 bp composed of five translated exons interrupted by four introns) with a conserved GT/AG exon-intron splicing rule. The putative non-translated exon predicted in the rockbream β-actin gene was much more homologous with those of teleostean β-actin genes than those of mammals. The 5'-upstream regulatory region isolated by genome walking displayed conserved and essential elements such as TATA, CArG and CAAT boxes in its proximal part, while several other immune- or stress-related motifs such as those for NF-kappa B, USF, HNF, AP-1 and C/EBP were in the distal part. Semi-quantitative RT-PCR assay results demonstrated that the rockbream β-actin transcripts were ubiquitously but different-tially expressed across the tissues of juveniles.
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등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Anderson K.V. / 2000 / Toll signaling pathways in the innate immune response / Curr. Opin. Immunol. 12 : 13 ~ 19
Andreassen / 2005 / Upregulation of estrogen receptor alpha and vitello-genin in eelpout (Zoarces viviparus) by waterborne exposure to 4-tert-octylphenol and 17beta-estradiol
Arukwe, A / 2006 / Toxicological housekeeping genes: do they really keep the house? / Environ. Sci. Technol. 40 : 7944 ~ 4949
Bayele / 2006 / Cis and trans regulation of hepcidin expression by upstream stimulatory factor / Blood (108) : 4237 ~ 4245
Brooks, C. / 2007 / Transgene activity following somatic transgenesis in Nile tilapia (Oreochromis niloticus) / J. Fish Biol. 70 : 234 ~ 247
Andreassen / 2005 / Upregulation of estrogen receptor alpha and vitello-genin in eelpout (Zoarces viviparus) by waterborne exposure to 4-tert-octylphenol and 17beta-estradiol
Arukwe, A / 2006 / Toxicological housekeeping genes: do they really keep the house? / Environ. Sci. Technol. 40 : 7944 ~ 4949
Bayele / 2006 / Cis and trans regulation of hepcidin expression by upstream stimulatory factor / Blood (108) : 4237 ~ 4245
Brooks, C. / 2007 / Transgene activity following somatic transgenesis in Nile tilapia (Oreochromis niloticus) / J. Fish Biol. 70 : 234 ~ 247
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