지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 303 - 310 (8page)
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We characterized a cytoplasmic actin gene locus in greenling Hexagrammos otakii (Scorpaeniformes). Genomic clones isolated from the greenling DNA library contained two homologous cytoplasmic actin gene copies (HObact2.1 and HObact2.2) in a tail-to-head orientation. Their gene structure is characterized by six translated exons and one non-translated exon. Exon-intron organization and the nucleotide sequences of the two actin gene isoforms are very similar. However, only the HObact2.1 isoform contains microsatellite-like, dinucleotide repeats in the 5′-flanking region (named HOms2002) and intron 1 following the non-translated exon 1 (named HOms769). One microsatellite locus (HOms769) was highly polymorphic while the other (HOms2002) was not. Based on bioinformatic analysis, different transcription factor binding motifs are related to stress and immune responses in the two actin isoforms. Semiquantitative and real-time reverse transcription-PCR assays showed that both isoform transcripts were detectable ubiquitously in all the tissues examined. However, the basal expression levels of each isoform varied across tissues. Overall, the two isoforms showed a similar, but not identical, expression pattern. Our data suggest that the cytoplasmic actin genes may be the result of a recent duplication event in the greenling genome, which has not experienced significant subfunctionalization in their housekeeping roles.
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참고문헌 (25)
참고문헌 신청
Anderson KV / 2000 / Toll signaling pathways in the innate immune response / Curr Opin Immunol 12 : 13 ~ 19
Chaponnier C / 2004 / Pathological situations characterized by altered actin isoform expression / J Pathol 204 : 386 ~ 395
Chen SL / 2009 / Isolation and characterization of polymorphic microsatellite loci from fat greenling(Hexagrammos otakii) / Conserv Genet 10 : 1429 ~ 1431
Cho YS / 2009 / Gene structure and differential modulation of multiple rockbream (Oplegnathus fasciatus) hepcidin isoforms resulting from different biological stimulations /
Cho YS / 2011 / Functional ability of cytoskeletal β-actin regulator to drive constitutive and ubiquitous expression of a fluorescent reporter throughout the life cycle of t
Chaponnier C / 2004 / Pathological situations characterized by altered actin isoform expression / J Pathol 204 : 386 ~ 395
Chen SL / 2009 / Isolation and characterization of polymorphic microsatellite loci from fat greenling(Hexagrammos otakii) / Conserv Genet 10 : 1429 ~ 1431
Cho YS / 2009 / Gene structure and differential modulation of multiple rockbream (Oplegnathus fasciatus) hepcidin isoforms resulting from different biological stimulations /
Cho YS / 2011 / Functional ability of cytoskeletal β-actin regulator to drive constitutive and ubiquitous expression of a fluorescent reporter throughout the life cycle of t
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