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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 26 - 35 (10page)
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In our previous study, we preliminarily demonstrated that Celluclast and Neutrase extracts exhibited the strongest H2O2-scavenging activities among five carbohydrases (Viscozyme, Celluclast, Termamyl, Ultraflo and AMG) and five proteases (Kojizyme, Alcalse, Flavourzyme Protamex and Neutrase) extracts. Thus, Celluclast and Neutrase extracts were selected for use in further experiments and were separated into four different molecular weight fractions (<5, 5-10, 10-30 and >30 kDa). Among them, the 5-10 kDa fraction showed the highest H2O2 scavenging activity. The 5-10 kDa fraction also strongly enhanced cell viability against H2O2-induced oxidative damage. Furthermore, the fraction reduced the proportion of apoptotic cells induced by H2O2, as demonstrated by decreased sub-G1 hypodiploid cells and decreased apoptotic body formation by flow cytometry. These results indicated that the 5-10 kDa fraction of the Celluclast and Neutrase extracts from S. coreanum exhibited strong antioxidant activity over H2O2-mediated cell damage in vitro.
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참고문헌 (25)
참고문헌 신청
Ahn G / 2007 / Antioxidant activities of phlorotannins puri-fied from Ecklonia cava on free radical scavenging using ESR and H2O2-mediated DNA damage / Eur Food Res Technol 2
Ames B / 1998 / prevent cancer and delay aging / Toxicol Lett 102 : 5 ~ 18
Athukorala Y / 2003 / Reduction of lipid pero-xidation and H2O2-mediated DNA damage by a red alga (Grateloupia filicina) methnolic extract / J Sci Food Agri 85 : 251 ~ 265
Athukorala Y / 2005 / Screening for angiotensin I-converting enzyme inhibitory activity of Ecklonia cava / J Food Sci Nutr 10 : 134 ~ 139
Bagchi D / 2000 / Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention / Toxicology 148 : 187 ~ 197
Ames B / 1998 / prevent cancer and delay aging / Toxicol Lett 102 : 5 ~ 18
Athukorala Y / 2003 / Reduction of lipid pero-xidation and H2O2-mediated DNA damage by a red alga (Grateloupia filicina) methnolic extract / J Sci Food Agri 85 : 251 ~ 265
Athukorala Y / 2005 / Screening for angiotensin I-converting enzyme inhibitory activity of Ecklonia cava / J Food Sci Nutr 10 : 134 ~ 139
Bagchi D / 2000 / Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention / Toxicology 148 : 187 ~ 197
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