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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 88 - 97 (10page)
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초록· 키워드
오류제보하기
Lipoproteins are complexes of lipids and specific apolipoproteins that are involved in lipid transport and redistribution among various tissues. In this study, we isolated full-length apolipoprotein cDNA sequences encoding apolipoprotein A-I (apoA-I), apoE, apoC-II, and apo-14 kDa in barred knifejaw, Oplegnathus fasciatus. In addition, we reconstructed phylogenetic trees and investigated mRNA tissue distributions. Alignment analyses of amino acid sequences revealed that secondary structures of the polypeptides apoA-I, apoE, and apoC-II in barred knifejaw are well conserved with their teleostean and mammalian counterparts in terms of characteristic tandem repetitive units forming amphipathic α-helices. Both the sequence alignment data and cleavage sites of apo-14 kDa indicated a clear differentiation between Percomorpha and Cypriniformes. Meanwhile, the phylogenetic trees of apolipoprotein sub-families suggested that the common ancestor prior to the split of the Actinopterygii (ray-finned fishes) and Sarcopterygii (tetrapods) would have possessed the primordial protein-encoding genes. Tissue distribution of each apolipoprotein transcript determined by semi-quantitative RT-PCR showed that barred knifejaw apoA-I transcripts were more or less ubiquitously expressed in the liver, intestines, brain, muscle, spleen, and kidney. The most striking difference from previous observations on barred knifejaw was the ubiquitous expression of apoE across all somatic tissues. Barred knifejaw apoC-II showed tissue-specific expression in the liver and intestines, while the liver and brain were the major sites of apo-14 kDa mRNA synthesis.
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등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Babin, P.J / 1997 / Both apolipoprotein E and A-I genes are present in a nonmammalian verteb-rate and are highly expressed during embryonic development / Proceedings of the N
Boguski, M.S / 1985 / Comparative analysis of repeated se-quences in rat apolipoproteins A-I, A-IV, and E / Proceedings of the National Academy of Sciences of the United Stat
Byrnes, L / 1987 / Chicken apolipoprotein A-I: cDNA sequence, tissue expression and evolution / Biochemical and Biophysical Research Communications 128 : 485 ~ 492
Concha, M.I / 2003 / Local expression of apolipoprotein A-I gene and a possible role for HDL in primary defence in the carp skin / Fish and Shellfish Immunology 14 : 259 ~ 27
Delcuve, G.P / 1992 / Expression of rainbow trout apolipoprotein A-I genes in liver and hepatocellular carcinoma / Journal of Lipid Research 33 : 251 ~ 262
Boguski, M.S / 1985 / Comparative analysis of repeated se-quences in rat apolipoproteins A-I, A-IV, and E / Proceedings of the National Academy of Sciences of the United Stat
Byrnes, L / 1987 / Chicken apolipoprotein A-I: cDNA sequence, tissue expression and evolution / Biochemical and Biophysical Research Communications 128 : 485 ~ 492
Concha, M.I / 2003 / Local expression of apolipoprotein A-I gene and a possible role for HDL in primary defence in the carp skin / Fish and Shellfish Immunology 14 : 259 ~ 27
Delcuve, G.P / 1992 / Expression of rainbow trout apolipoprotein A-I genes in liver and hepatocellular carcinoma / Journal of Lipid Research 33 : 251 ~ 262
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