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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 845 - 857 (13page)
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Background and Objectives : Korean mistletoe lectin (Viscum album coloratum agglutinin, VCA) and bee venom (BV) have been reported to induce apoptosis in various cancer cell lines in vitro and to antitumor activity against a variety of tumors in animal models. However, the comparative study of VCA and BV on the anti-cancer effect and mechanisms of action has not been determined. In this study, it was examined in a human hepatocellular carcinoma cell line, Hep G2 cells.
Methods : Cytotoxic effects of VCA and BV on Hep G2 cells were determined by 3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide (MTT) assay in vitro. The apoptotic cell death was then confirmed by propidium iodide staining and DNA fragmentation analysis. The mechanisms of action was examined by the expression of anti-apoptotic proteins and activation of mitogen-activated protein kinases. The involvement of kinase was examined in VCA or BV-induced apoptosis by using kinase inhibitors.
Results : VCA and BV killed Hep G2 cells in a time and dose-dependent manner. Treatment of Hep G2 cells with VCA activated poly (ADP-ribose) polymerase-1 (PARP-1) known as a marker of apoptosis, and mitogen-activated protein kinases signaling pathways including MAPK/ERK, p38 MAPK and JNK. BV also activated PARP-1, MAPK/ERK, p38 MAPK but not JNK. The expression level of anti-apoptotic molecule, Bcl-X, was decreased by VCA treatment but not BV. Finally, the phosphorylation level of ERM proteins involved in the cytoskeleton homeostasis was decreased by both stimuli. VCA-induced apoptosis was partially inhibited by in the presence of JNK and p38 inhibitor but, BV by only p38 inhibitor.
Conclusions : VCA induced apoptosis is dependent on the activation of p38 and JNK and BV-induced apoptosis is mediated by p38 activation in Hep G2 cells.
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참고문헌 (25)
참고문헌 신청
Bocci V / 1993 / Mistletoe (viscum album) lectins as cytokine inducers and immunoadjuvant in tumor therapy. A review / J Biol Regul Homeost Agents 7 (1) : 1 ~ 6
Branas P / 2000 / Treatments for fatigue in multiple sclerosis : a rapid and systematic review / Health Technol Assess 4 (27) : 1 ~ 61
Deregnaucourt C / 2000 / Bee venom phospholopase A2 induces stage-specific growth arrest of the intraerythrocytic Plasmodium falciparum via modifications of human serum compo
Fischer S / 1997 / Oligoclonal in vitro response of CD4 T cells to vesicles of mistletoe extracts in mistletoe-treated cancer patients / Cancer Immunol Immunother 44 (3) : 15
Gerst JE / 1987 / Inhibition by melittin and fluphenazine of melanotropin receptor function and adenylate cyclase in M2R melanoma cell membranes / Endocrinology 121 (5) : 17
Branas P / 2000 / Treatments for fatigue in multiple sclerosis : a rapid and systematic review / Health Technol Assess 4 (27) : 1 ~ 61
Deregnaucourt C / 2000 / Bee venom phospholopase A2 induces stage-specific growth arrest of the intraerythrocytic Plasmodium falciparum via modifications of human serum compo
Fischer S / 1997 / Oligoclonal in vitro response of CD4 T cells to vesicles of mistletoe extracts in mistletoe-treated cancer patients / Cancer Immunol Immunother 44 (3) : 15
Gerst JE / 1987 / Inhibition by melittin and fluphenazine of melanotropin receptor function and adenylate cyclase in M2R melanoma cell membranes / Endocrinology 121 (5) : 17
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