지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Bee venom (BV), which is extracted from honeybees, has been used in traditional Korean medical therapy. Glutamate-mediated excitotoxicity contributes to neuronal death in neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) or Alzheimer's disease (AD). This study is to investigate the effect of BV on glutamate-induced neurotoxicity on NSC-34 motor neuron cells. To determine the viability of motor neuronal cells, we performed with MTT assays in glutamate-treated NSC-34 cell with BV or without. For the measurement of oxidative stress, DCF assay was used in glutamate-treated NSC-34 motor neuronal cells with BV or without. To investigate the molecular mechanism of BV against glutamate-mediated neurotoxicity in NSC-34 cells, western blot analysis was used. Glutamate significantly decreased cell viability by glutamate dose- or treatment time-dependent manner in NSC-34 cells. However, BV pre-treatment dramatically inhibited glutamate-induced neuronal cell death. Furthermore, we found that BV increased the expression of Bcl-2 protein that is anti-apoptotic protein and reduced the generation of oxidative stress. BV has a neuroprotective role against glutamate neurotoxicity by an increase of anti-apoptotic protein. It suggests that BV may be useful for the reduction of neuronal cell death in neuronal disease models.
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참고문헌 (24)
참고문헌 신청
Foran, E / 2009 / Glutamate transporters and the excitotoxic path to motor neuron degeneration in amyotrophic lateral sclerosis / Antioxid Redox Signal 11:1587
Butcher, S. P / 1987 / In vivo studies on the extracellular, and veratrine-releasable, pools of endogenous amino acids in the rat striatum: effects of corticostriatal deafferentation and kainic acid lesion / J. Neurochem 48:713
Abe, K. / 2003 / Agmatine induces glutamate release and cell death in cultured rat cerebellar granule neurons / Brain Research 990:165
Kihara, T. / 2002 / Protective effect of dopamine D2 agonists in cortical neurons via the phosphatidylinositol 3 kinase cascade / J. Neurosci. Res 70:274
Harris, M. E / 1996 / Amyloid beta peptide (25–35) inhibits Na+-dependent glutamate uptake in rat hippocampal astrocyte cultures / J. Neurochem 67:277
Butcher, S. P / 1987 / In vivo studies on the extracellular, and veratrine-releasable, pools of endogenous amino acids in the rat striatum: effects of corticostriatal deafferentation and kainic acid lesion / J. Neurochem 48:713
Abe, K. / 2003 / Agmatine induces glutamate release and cell death in cultured rat cerebellar granule neurons / Brain Research 990:165
Kihara, T. / 2002 / Protective effect of dopamine D2 agonists in cortical neurons via the phosphatidylinositol 3 kinase cascade / J. Neurosci. Res 70:274
Harris, M. E / 1996 / Amyloid beta peptide (25–35) inhibits Na+-dependent glutamate uptake in rat hippocampal astrocyte cultures / J. Neurochem 67:277
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