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논문 기본 정보

자료유형
학술저널
저자정보
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대한한방내과학회 대한한방내과학회지 대한한방내과학회지 제31권 제3호
발행연도
2010.1
수록면
650 - 666 (17page)

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Objectives : In this study, the author tried to investigate whether wood vinegar produced from Morus alba (MA) significantly affects the increase in airway epithelial mucosubstances and hyperplasia of tracheal goblet cells of rats, and in vitro airway mucin secretion and PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production / gene expression from human airway epithelial cells. Materials and Methods : For the in vivo experiment, the author induced hypersecretion of airway mucus and goblet cell hyperplasia by exposure of rats to SO2 over 3 weeks. Effect of orally-administered MA over 2 weeks on increase in airway epithelial mucosubstances from tracheal goblet cells of rats and hyperplasia of goblet cells were assessed using histopathological analysis after staining the epithelial tissue with alcian blue. For the in vitro experiment, confluent RTSE cells were chased for 30 min in the presence of MA to assess the effect of MA on mucin secretion by enzyme-linked immunosorbent assay (ELISA). Also, effects of MA on PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production and gene expression from human airway epithelial cells (NCI-H292) were investigated. Confluent NCI-H292 cells were pretreated for 30 min in the presence of MA and treated with PMA (10 ng/㎖), EGF (25 ng/㎖) or TNF-alpha (0.2 ㎚) for 24 hrs, to assess both effects of MA on PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production by enzyme-linked immunosorbent assay (ELISA) and gene expression by reverse transcription-polymerase chain reaction (RT-PCR). Possible cytotoxicities of MA in vitro were assessed by examining LDH release from RTSE cells and the rate of survival and proliferation of NCI-H292 cells. In vivo liver and kidney toxicities of MA were evaluated by measuring serum GOT/GPT activities and serum BUN/creatinine concentrations of rats after administering MA orally. Results :1. MA decreased the amount of intraepithelial mucosubstances of rats exposed to sulfur dioxide inhalationally. 2. MA decreased in vitro mucin secretion from cultured RTSE cells. 3. MA significantly inhibited PMA-, EGF-, and TNF-alpha-induced MUC5AC mucin productions and the expression levels of MUC5AC mRNA from NCI-H292 cells. 4. MA did not show either in vitro or in vivo hepatic or renal toxicities. Conclusion : The results from this study suggests that MA can regulate the secretion, production and gene expression of airway mucin observed in diverse respiratory diseases accompanied by mucus hypersecretion and does not show in vivo toxicity to liver and kidney functions after oral administration. Effects of MA should be further studied using animal experimental models that simulate the diverse pathophysiology of respiratory diseases via future research.
#respiratory system #mucus #mucin #secretion #wood vinegar produced from Morus alba #MA)

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참고문헌 (27)

참고문헌 신청
Cha JY / 2001 / Effect of Stem Bark Extract From morus alba and Cudrania tricuspidata on the Concentrations of Lipid and Tissue Lipid Peroxidation in the Cholesterol-Fed Rat google schola Culpitt SV / 2005 / Sputum matrix metalloproteases: comparison between chronic obstructive pulmonary disease and asthma / Respir Med 99 (6) : 703 ~ 710 google schola Freshny / 1994 / Measurement of viability and cytotoxicity. In: Culture of animal cells / Willey-Liss : 288 ~ google schola Gleich GJ / 1990 / The eosinophil and bronchial asthma: Current understanding / J. Allergy Clin. Immunol. 85 : 422 ~ 436 google schola Harkema JR / 1992 / In vivo Effect of endotoxin on intraepithelial mucosubstances in rat pulmonary airways: quantitative histochemistry / Am. J. Pathol. : 141307 ~ 141331 google schola

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