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학제간연구 과학기술학 뇌인지과학 기술정책 기타 복합학

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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
대한한방소아과학회 대한한방소아과학회지 대한한방소아과학회지 제28권 제2호
발행연도
2014.1
수록면
56 - 71 (16page)

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ObjectivesIn this study, the author tried to investigate whether piryongbang-gamgil-tang (PGGT) significantly affect in vitroairway mucin secretion, PMA- or EGF- or TNF-α-induced MUC5AC mucin production / gene expression from humanairway epithelial cells and increase in airway epithelial mucosubstances and hyperplasia of tracheal goblet cells of rats. Materials and MethodsFor in vitro experiment, confluent RTSE cells were chased for 30 min in the presence of PGGT to assess the effectof PGGT on mucin secretion by enzyme-linked immunosorbent assay (ELISA). Also, effect of PGGT on PMA- or EGForTNF-α-induced MUC5AC mucin production and gene expression from human airway epithelial cells (NCI-H292) wereinvestigated. Confluent NCI-H292 cells were pretreated for 30 min in the presence of PGGT and treated with PMA(10 ng/ml) or EGF (25 ng/ml) or TNF-α (0.2 nM) for 24 hrs, to assess both effect of PGGT on PMA- or EGF- or TNF-α-inducedMUC5AC mucin production by ELISA and gene expression by reverse transcription-polymerase chain reaction (RT-PCR). For in vivo experiment, the author induced hypersecretion of airway mucus and goblet cell hyperplasia by exposureof rats to SO2 during 3 weeks. Effect of orally-administered PGGT during 2 weeks on increase in airway epithelialmucosubstances from tracheal goblet cells of rats and hyperplasia of goblet cells were assesed by using histopathologicalanalysis after staining the epithelial tissue with alcian blue. Possible cytotoxicities of PGGT in vitro were assessed by examining LDH release from RTSE cells and the rateof survival and proliferation of NCI-H292 cells. In vivo liver and kidney toxicities of PGGT were evaluated by measuringserum GOT/GPT activities and serum BUN/creatinine concentrations of rats after administering PGGT orally. Results(1) PGGT did not affect in vitro mucin secretion from cultured RTSE cells. (2) PGGT significantly inhibitedPMA-, EGF-, and TNF-α-induced MUC5AC mucin productions and the expression levels of MUC5AC mRNA fromNCI-H292 cells. (3) PGGT decreased the amount of intraepithelial mucosubstances and showed the tendency ofexpectorating airway mucus already produced. (4) PGGT increased LDH release from RTSE cells. However, PGGTdid not show in vivo liver and kidney toxicities and cytotoxicity to NCI-H292 cells. ConclusionThe result from this study suggests that PGGT can regulate the production and gene expression of airway mucinobserved in diverse respiratory diseases accompanied by mucus hypersecretion and do not show in vivo toxicity toliver and kidney functions after oral administration. Effect of PGGT with their components should be further studiedusing animal experimental models that reflect the diverse pathophysiology of respiratory diseases through future investigations.
#Airway #Mucin #Gene #Piryongbanggamgil-tang #PGGT)

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참고문헌 (54)

참고문헌 신청
Song KS / 2003 / Interleukin-1beta and tumor necrosis factor-alpha induce MUC5AC overexpression through a mechanism involving ERK/p38 mitogen-activated protein kinases-MSK1-CREB activation in human airway epithelial cells / J Biol Chem 278(26):23243~23250 google schola Karlinsey J / 1989 / Simultaneous purification of DNA and RNA from small numbers of eukaryotic cells / Anal Biochem 180(2):303~306 google schola Pon DJ / 1994 / Hyperplastic effects of aerosolized sodium metasulfite on rat airway mucus-secretory epithelial cells / Can J Physiol Pharmacol 72(9):1025~1030 google schola Harkema JR / 1992 / In vivo effect of endotoxin on intraepithelial mucosubstances in rat pulmonary airways : quantitative histochemistry / Am J Pathol 141(2):307~317 google schola George JA / 1985 / An immunohistochemical characterization of rhesus monkey respiratoty secretions using monoclonal antibodies / Am Rev Resp Dis 132(3):556~563 google schola

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