지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 685 - 695 (11page)
이용수
초록· 키워드
오류제보하기
Objectives : In this study, the author tried to investigate whether Geonpye-tang(GPT) significantly affect PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production and gene expression from human airway epithelial cells.
Materials and Methods : Effect of the agent on PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production and gene expression from human airway epithelial cells (NCI-H292) were investigated. Confluent NCI-H292 cells were pretreated for 30 min in the presence of GPT and treated with PMA (10ng/ml) or EGF (25ng/ml) or TNF-alpha (0.2nM), to assess both effect of the agent on PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production by enzyme-linked immunosorbent assay (ELISA) and gene expression by reverse transcription-polymerase chain reaction (RT-PCR). Possible cytotoxicity of the agent was assessed by examining the rate of survival and proliferation of NCI-H292 cells after treatment of agent during 72 hrs (SRB assay).
Results : (1) GPT significantly inhibit PMA-induced and EGF-induced MUC5AC mucin productions from NCI-H292 cells. However, GPT did not affect TNF-alpha-induced MUC5AC mucin production ; (2) GPT significantly inhibit the expression levels of PMA- or EGF- or TNF-alpha-induced MUC5AC gene in NCI-H292 cells ; (3) GPT did not show significant cytotoxicity to NCI-H292 cells.
Conclusion : This result suggests that GPT can affect the production and gene expression of respiratory mucin observed in diverse respiratory diseases accompanied by mucus hypersecretion. This can explain the traditional use of GPT in oriental medicine. Effect of GPT with their components should be further investigated using animal experimental models that reflect pathophysiology of airway diseases through future studies.
목차
등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Choi SB / 1985 / A study of effects of Smasoeum on the analgesia, Antipyresis and O3 posisoned lung in rats and mice / K. H. Univ. O. Med. J 8 : 139 ~ 148
Culpitt SV / 2005 / Sputum matrix metalloproteases: comparison between chronic obstructive pulmonary disease and asthma / Respir Med 99 (6) : 703 ~ 710
Frigas, E / 1981 / Elevated levels of the eosinophil granule major basic protein in the sputum of patients with bronchial asthma. Mayo Clin / Proc 56 : 345 ~ 353
Gleich, G.J / 1990 / The eosinophil and bronchial asthma: Current understanding / J. Allergy Clin. Immunol 85 : 422 ~ 436
Karlinsey, J / 1989 / Simultaneous purification of DNA and RNA from small numbers of eukaryotic cells / Anal. Biochem 180 (2) : 303 ~ 306
Culpitt SV / 2005 / Sputum matrix metalloproteases: comparison between chronic obstructive pulmonary disease and asthma / Respir Med 99 (6) : 703 ~ 710
Frigas, E / 1981 / Elevated levels of the eosinophil granule major basic protein in the sputum of patients with bronchial asthma. Mayo Clin / Proc 56 : 345 ~ 353
Gleich, G.J / 1990 / The eosinophil and bronchial asthma: Current understanding / J. Allergy Clin. Immunol 85 : 422 ~ 436
Karlinsey, J / 1989 / Simultaneous purification of DNA and RNA from small numbers of eukaryotic cells / Anal. Biochem 180 (2) : 303 ~ 306
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