지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제47권 제2호
- 발행연도
- 2017.1
- 수록면
- 116 - 131 (16page)
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초록· 키워드
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Purpose: The entry of bacteria or harmful substances through the epithelial seal of human gingival keratinocytes (HGKs) in the junctional epithelium (JE) is blocked by specialized intercellular junctions such as E-cadherin junctions (ECJs). However, the influence of roughened substrates, which may occur due to apical migration of the JE, root planing, or peri-implantitis, on the development of the ECJs of HGKs remains largely unknown.
Methods: HGKs were cultured on substrates with varying levels of roughness, which were prepared by rubbing hydrophobic polystyrene dishes with silicon carbide papers. The activity of c-Jun N-terminal kinase (JNK) was inhibited with SP600125 or by transfection with JNK short hairpin RNA. The development of intercellular junctions was analyzed using scanning electron microscopy or confocal laser scanning microscopy after immunohistochemical staining of the cells for E-cadherin. The expression level of phospho-JNK was assessed by immunoblotting.
Results: HGKs developed tight intercellular junctions devoid of wide intercellular gaps on smooth substrates and on rough substrates with low-nanometer dimensions (average roughness [Ra]=121.3±13.4 nm), although the ECJs of HGKs on rough substrates with low-nanometer dimensions developed later than those of HGKs on smooth substrates. In contrast, HGKs developed short intercellular junctions with wide intercellular gaps on rough substrates with mid- or high-nanometer dimensions (Ra=505.3±115.3 nm, 867.0±168.6 nm). Notably, the stability of the ECJs was low on the rough substrates, as demonstrated by the rapid destruction of the cell junction following calcium depletion. Inhibition of JNK activity promoted ECJ development in HGKs. JNK was closely associated with cortical actin in the regulation of ECJs in HGKs.
Conclusions: These results indicate that on rough substrates with nanometer dimensions, the ECJs of HGKs develop slowly or defectively, and that this effect can be reversed by inhibiting JNK.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Mengistu M / 2011 / Fluid shear stress-induced JNK activity leads to actin remodeling for cell alignment / J Cell Physiol 226:110~121
Barkmeier WW / 2009 / Effect of enamel etching time on roughness and bond strength / Oper Dent 34 : 217 ~ 222
Bosshardt DD / 2005 / The junctional epithelium : from health to disease / J Dent Res 84 : 9 ~ 20
Hamel M / 2006 / Active stress kinases in proliferat
Hara AT / 2016 / Dental surface texture characterization based on erosive tooth wear processes / J Dent Res 95 : 537 ~ 542
Barkmeier WW / 2009 / Effect of enamel etching time on roughness and bond strength / Oper Dent 34 : 217 ~ 222
Bosshardt DD / 2005 / The junctional epithelium : from health to disease / J Dent Res 84 : 9 ~ 20
Hamel M / 2006 / Active stress kinases in proliferat
Hara AT / 2016 / Dental surface texture characterization based on erosive tooth wear processes / J Dent Res 95 : 537 ~ 542
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