지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 204 - 211 (8page)
이용수
초록· 키워드
오류제보하기
Background : Since metallo-β-lactamase (MBL)-producing isolates can hydrolyze carbapenem and also easily transfer the resistance genes to other bacteria, a rapid and accurate detection of MBL has become very important. We evaluated the utility of Mueller Hinton agar (MHA) biplate containing
dipicolinic acid (DPA) as a screening method to detect IMP-1 and VIM-2 type MBL-producing isolates.
Methods : Based on our preliminary tests using various concentrations of DPA, 200 and 300 μg/mL concentration of DPA were chosen for further study. Bacterial lawns were grown on MHA biplate, one half of which contained DPA while the other did not. The inhibition zone around the imipenem (IPM)
disk on both sides of this plate was compared. The stability of DPA in the stored DPA-MHA biplate was also evaluated during three months using two MBL- and one non-MBL-producing isolates.
Results : When the criterion of a ≥7 mm increase of inhibition zone around the IPM disk on the MHA containing DPA compared to MHA without DPA was used, the sensitivities and specificities were 94.7% and 97.6% for 200 μg/mL DPA-MHA biplate, and 98.2% and 97.6% for 300 μg/mL DPAMHA
biplate, respectively. The activity of the DPA in this biplate was stable for three months.
Conclusions : Assays using DPA 300-MHA biplate were highly sensitive and specific for the detection of IMP-1 and VIM-2 type MBL-producing
bacteria. In addition, it is easy to perform; so, it may be useful to apply this method for detection of IMP-1 and VIM-2 type MBL in clinical
laboratories.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Ambler RP / 1980 / The structure of beta-lactamases / Philos Trans R Soc Lond B Biol Sci 289 : 321 ~ 331
Arakawa Y / 2000 / Convenient test for screening metallo-β-lactamase-producing gram-negative bacteria by thiol compounds / J Clin Microbiol 38 : 40 ~ 43
Bush K / 1995 / A functional classification scheme for β-lactamases and its correlation with molecular structure / Antimicrob Agents Chemother 39 : 1211 ~ 1233
Castanheira M / 2004 / Molecular characterization of a β-lactamase gene, blaGIM-1, encoding a new subclass of metallo-β-lactamase / Antimicrob Agents Chemother 48 : 4654 ~
Chu YW / 2005 / EDTA susceptibility leading to false detection of metallo-β-lactamase in Pseudomonas aeruginosa by Etest and an imipenem-EDTA disk method / Int J Antimicrob
Arakawa Y / 2000 / Convenient test for screening metallo-β-lactamase-producing gram-negative bacteria by thiol compounds / J Clin Microbiol 38 : 40 ~ 43
Bush K / 1995 / A functional classification scheme for β-lactamases and its correlation with molecular structure / Antimicrob Agents Chemother 39 : 1211 ~ 1233
Castanheira M / 2004 / Molecular characterization of a β-lactamase gene, blaGIM-1, encoding a new subclass of metallo-β-lactamase / Antimicrob Agents Chemother 48 : 4654 ~
Chu YW / 2005 / EDTA susceptibility leading to false detection of metallo-β-lactamase in Pseudomonas aeruginosa by Etest and an imipenem-EDTA disk method / Int J Antimicrob
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