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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 240 - 247 (8page)
이용수
초록· 키워드
오류제보하기
Background: The gold standard for antinuclear antibody (ANA) screening is the indirect immunofluorescence (IIF) assay with human epithelial cells (HEp-2). However, a number of substantial disadvantages of manual IIF assays have highlighted the need for the automation and standardization of fluorescent ANA (FANA) testing. We evaluated the performance of EUROPattern Suite (Euroimmun AG, Germany), an automated FANA image analyzer, with regard to ANA detection and pattern recognition compared with conventional manual interpretation using the fluorescence microscopic IIF assay.
Methods: A total of 104 samples including 70 ANA-positive sera and 34 ANA-negative sera collected from September to October 2015 were included. The sensitivity, specificity, and pattern recognition function were evaluated to determine the performance of EUROPattern Suite compared with the manual IIF assay results.
Results: The sensitivity and specificity of EUROPattern Suite for ANA detection were 94.3% and 94.1%, respectively. The concordance rate between the two methods was 94.2%. For pattern recognition, 45.7% of the samples were assigned identical ANA patterns including simple and mixed. When major pattern matching was considered, 83.7% (41/49) and 95.2% (20/21) of the samples with simple and mixed patterns, respectively, showed concordant results between the two methods.
Conclusions: EUROPattern Suite, an automated FANA image analyzer, provides a viable option for distinguishing between positive and negative results, although the ability to assign specific patterns is insufficient to replace manual microscopic interpretation. This automated system may increase efficiency in laboratories, in which a large number of samples need to be processed.
목차
등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Bizzaro N / 2014 / Automated antinuclear immunofluorescence antibody screening: a comparative study of six computer-aided diagnostic systems / Autoimmun Rev 13 : 292 ~ 298
Chan EK / 2015 / Report of the First International Consensus on Standardized Nomenclature of Antinuclear Antibody HEp-2 Cell Patterns 2014-2015 / Front Immunol 6 : 412 ~
Damoiseaux J / 2016 / International consensus on ANA patterns (ICAP): the bumpy road towards a consensus on reporting ANA results / Auto Immun Highlights 7 : 1 ~
Ganapathy V / 2004 / Autoimmunity to the nuclear autoantigen DFS70 (LEDGF): what exactly are the autoantibodies trying to tell us? / Arthritis Rheum 50 : 684 ~ 688
Gerlach S / 2015 / Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology / J Immunol Res
Chan EK / 2015 / Report of the First International Consensus on Standardized Nomenclature of Antinuclear Antibody HEp-2 Cell Patterns 2014-2015 / Front Immunol 6 : 412 ~
Damoiseaux J / 2016 / International consensus on ANA patterns (ICAP): the bumpy road towards a consensus on reporting ANA results / Auto Immun Highlights 7 : 1 ~
Ganapathy V / 2004 / Autoimmunity to the nuclear autoantigen DFS70 (LEDGF): what exactly are the autoantibodies trying to tell us? / Arthritis Rheum 50 : 684 ~ 688
Gerlach S / 2015 / Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology / J Immunol Res
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