지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 35 - 40 (6page)
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초록· 키워드
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Background: Final diagnosis of paroxysmal nocturnal hemoglobinuria (PNH) may take years demanding a quick diagnosis measure. We used the facts that PNH cells are damaged in acid, and reagents for measuring reticulocytes in Coulter DxH800 (Beckman Coulter, USA) are weakly acidic and hypotonic, to create a new PNH screening marker.
Methods: We analyzed 979 complete blood counts (CBC) data from 963 patients including 57 data from 44 PNH patients. Standard criteria for PNH assay for population selection were followed: flow cytometry for CD55 and CD59 on red blood cells (RBCs) to a detection level of 1%; and fluorescent aerolysin, CD24 and CD15 in granulocytes to 0.1%. Twenty-four PNH minor clone-positive samples (minor-PNH+) were taken, in which the clone population was <5% of RBCs and/or granulocytes. Excluding PNH and minor-PNH+ patients, the population was divided into anemia, malignancy, infection, and normal groups. Parameters exhibiting a distinct demarcation between PNH and non-PNH groups were identified, and each parameter cutoff value was sought that includes the maximum [minimum] number of PNH [non-PNH] patients.
Results: Cutoff values for 5 selected CBC parameters (MRV, RDWR, MSCV, MN-AL2-NRET, and IRF) were determined. Positive rates were: PNH (86.0%), minor-PNH+ (33.3%), others (5.0%), anemia (13.4%), malignancy (5.3%), infection (3.7%), normal (0.0%); within anemia group, aplastic anemia (40.0%), immune hemolytic anemia (11.1%), iron deficiency anemia (1.6%). Sensitivity (86.0%), specificity (95.0%), PPV (52.1%), and NPV (99.1%) were achieved in PNH screening.
Conclusion: A new PNH screening marker is proposed with 95% specificity and 86% sensitivity. The flag identifies PNH patients, reducing time to final diagnosis by flow cytometry.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Borowitz MJ / 2010 / Guidelines for the diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria and related disorders by flow cytometry / Cytometry B ClinCytom 78 :
Brodsky RA / 2006 / PIG-A mutations in paroxysmal nocturnal hemoglobinuria and in normal hematopoiesis / Leuk Lymphoma 47 : 1215 ~ 1221
Brodsky RA / 2008 / Multicenter phase 3 study of the complement inhibitor eculizumab for the treatment of patients with paroxysmal nocturnal hemoglobinuria / Blood 111 : 184
Brodsky RA / 2009 / How I treat paroxysmal nocturnal hemoglobinuria / Blood 113 : 6522 ~ 6527
Broseus J / 2010 / Evaluation of mean sphered corpuscular volume for predicting hereditary spherocytosis / Int J Lab Hematol 32 : 519 ~ 523
Brodsky RA / 2006 / PIG-A mutations in paroxysmal nocturnal hemoglobinuria and in normal hematopoiesis / Leuk Lymphoma 47 : 1215 ~ 1221
Brodsky RA / 2008 / Multicenter phase 3 study of the complement inhibitor eculizumab for the treatment of patients with paroxysmal nocturnal hemoglobinuria / Blood 111 : 184
Brodsky RA / 2009 / How I treat paroxysmal nocturnal hemoglobinuria / Blood 113 : 6522 ~ 6527
Broseus J / 2010 / Evaluation of mean sphered corpuscular volume for predicting hereditary spherocytosis / Int J Lab Hematol 32 : 519 ~ 523
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