지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 28 - 35 (8page)
이용수
초록· 키워드
오류제보하기
Background: The indirect basophil activation test using flow cytometry is a promising tool for autoimmune urticaria diagnosis. We aimed to identify better donor basophils (from atopic vs. non-atopic donors and interleukin-3 primed vs. unprimed basophils) and improve basophil identification and activation markers (eotaxin CC chemokine receptor-3 [CCR3] vs. CD123 and CD63 vs. CD203c).
Methods: Donor basophils were obtained from non-atopic and atopic group O donors. Positive control sera were artificially prepared to simulate autoimmune urticaria patients’ sera. Patient sera were obtained from nine children with chronic urticaria. Assay sensitivity was compared among each variation by using positive control sera (n=21), applying cutoff values defined from negative control sera (n=20).
Results: For basophil identification, a combination of CCR3 and CD123 markers revealed a higher correlation with automated complete blood count (r=0.530) compared with that observed using CD123 (r=0.498) or CCR3 alone (r=0.195). Three activation markers on the atopic donor basophils attained 100% assay sensitivity: CD203c on unprimed basophils, CD63+CD203+ or CD63 alone on primed basophils; however, these markers on the non-atopic donor basophils attained lower assay sensitivity.
Conclusions: For basophil identification markers, a combination of CD123 and CCR3 is recommended, while CD123 alone may be used as an alternative. Donor basophils should be obtained from an atopic donor. For basophil activation markers, either CD203c alone on unprimed basophils or CD203c and CD63 on primed basophils are recommended, while CD63 alone on primed basophils may be used as an alternative.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Marrouche N / 2012 / Childhood urticaria / Curr Opin Allergy Clin Immunol 12:485~490
Fiebiger E / 1998 / Anti-FcepsilonRIalpha autoantibodies in autoimmune-mediated disorders. Identification of a structure-function relationship / J Clin Invest 101:243~251
Irinyi B / 2007 / Clinical and laboratory examinations in the subgroups of chronic urticaria / Int Arch Allergy Immunol 144:217~225
Ebo DG / 2008 / Basophil activation test by flow cytometry: present and future applications in allergology / Cytometry B Clin Cytom 74:201~210
Marone G / 1994 / The clinical relevance of basophil releasability / J Allergy Clin Immunol 94:1293~1303
Fiebiger E / 1998 / Anti-FcepsilonRIalpha autoantibodies in autoimmune-mediated disorders. Identification of a structure-function relationship / J Clin Invest 101:243~251
Irinyi B / 2007 / Clinical and laboratory examinations in the subgroups of chronic urticaria / Int Arch Allergy Immunol 144:217~225
Ebo DG / 2008 / Basophil activation test by flow cytometry: present and future applications in allergology / Cytometry B Clin Cytom 74:201~210
Marone G / 1994 / The clinical relevance of basophil releasability / J Allergy Clin Immunol 94:1293~1303
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Basophil Activation Test for Chronic Urticaria
Annals of Laboratory Medicine
2016 .01
-
혈중 호염기구 수에 따른 성인 천식의 임상 양상 분석 및 호기 응축물 내 사이토카인과의 연관성 분석
Allergy Asthma & Respiratory Diseases
2020 .01
-
Basophil Activation Tests: A Diagnostic Break-Through in Opiate Allergy
Allergy, Asthma & Immunology Research
2015 .01
-
Microarray-Based Multivariate Analysis of the Effectiveness of Sublingual Immunotherapy for Cedar Pollinosis
Allergy, Asthma & Immunology Research
2018 .01
-
An Ofloxacin-Induced Anaphylaxis through an IgG4-Mediated but Not IgE-Mediated Basophil Activation Mechanism
Acute and Critical Care
2017 .08
-
Short-, Intermediate-, and Long-Term Changes in Basophil Reactivity Induced by Venom Immunotherapy
Allergy, Asthma & Immunology Research
2016 .01
-
Reduced Venous Blood Basophil Count and Anxious Depression in Patients with Major Depressive Disorder
PSYCHIATRY INVESTIGATION
2016 .01
-
Basophil Activation Test Based on CD203c Expression in the Diagnosis of Fish Allergy
Allergy, Asthma & Immunology Research
2020 .01
-
Pollen/Fruit Syndrome: Clinical Relevance of the Cypress Pollen Allergenic Gibberellin-Regulated Protein
Allergy, Asthma & Immunology Research
2019 .01
-
Extrinsic Acquisition of CD80 by Antigen-Specific CD8⁺ T Cells Regulates Their Recall Immune Responses to Acute Viral Infection
Immune Network
2019 .08
-
Risk Factors Predicting Severe Asthma Exacerbations in Adult Asthmatics: A Real-World Clinical Evidence
Allergy, Asthma & Immunology Research
2021 .01
-
CD80CD86 deficiency disrupts regulator T cell homeostasis and induces autoimmune alopecia
한국실험동물학회 학술발표대회 논문집
2016 .08
-
CD5 Expression Dynamically Changes During the Differentiation of Human CD8+ T Cells Predicting Clinical Response to Immunotherapy
Immune Network
2023 .08
-
Leukemia propagating cells in Philadelphia chromosome-positive ALL: a resistant phenotype with an adverse prognosis
Blood Research
2018 .01
-
Changes in serum levels of lipopolysaccharides and CD26 in patients with Crohn’s disease
Intestinal research
2017 .01
-
Two Distinct Subsets Are Identified from the Peritoneal Myeloid Mononuclear Cells Expressing both CD11c and CD115
Immune Network
2019 .06
-
The Role of CD4 T Cell Help in CD8 T Cell Differentiation and Function During Chronic Infection and Cancer
Immune Network
2023 .10
-
Stem Cell Markers Predict the Response to Sorafenib in Patients with Hepatocellular Carcinoma
Gut and Liver
2019 .01
-
Effect of Peripheral Blood CD4 + CD25 + Regulatory T Cell on Postoperative Immunotherapy for Patients with Renal Carcinoma
Asian Pacific journal of cancer prevention : APJCP
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0