Assessment of Di (2-ethylhexyl) Phthalate Exposure by Urinary Metabolites as a Function of Sampling Time

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자료유형: 학술저널

저자정보: 박문서 양윤정 김상연 이용필 홍연표

저널정보: 대한예방의학회 예방의학회지 예방의학회지 제43권 제4호

발행연도: 2010.1

수록면: 301 - 308 (8page)

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Objectives: In most DEHP exposure assessment studies, single spot urine sample was used. It could not compare the exposure level among studies. Therefore, we are going to represent the necessity of selection of proper sampling time of spot urine for assessing the environmental DEHP exposure, and the association urinary DEHP metabolites with steroid hormones. Methods: We collected urine and plasma from 25 men. The urine sampling times were at the end of the shift (post-shift)and the next morning before the beginning of the shift (pre-shift). Three metabolites of DEHP {mono(2-ethylhexyl)phthalate [MEHP], mono-(2-ethyl-5-hydroxyhexyl)phthalate [MEHHP], and mono(2-ethyl-5-oxohexyl)phthalate [MEOHP]}in urine were analyzed by HPLC/MS/MS. Plasma luteinzing hormone, follicle stimulating hormone, testosterone, and 17β-estradiol were measured at pre-shift using a ELISA kit. A log-transformed creatinine-adjusted urinary MEHP, MEHHP, and MEOHP concentration were compared between the post- and pre-shift. The Pearson’s correlation was calculated to assess the relationships between log-transformed urinary MEHP concentrations in pre-shift urine and hormone levels. Results: The three urinary metabolite concentrations at post-shift were significantly higher than the concentrations in the pre-shift (p<0.0001). The plasma hormones were not significantly correlated with log-transformed creatinine - adjusted DEHP metabolites. Conclusions: To assess the environmental DEHP exposure, it is necessary to select the urine sampling time according to the study object. There were no correlation between the concentration of urinary DEHP metabolites and serum hormone levels.

#DEHP metabolites #Exposure #Sampling time #Steroid hormones #Urine #HPLC/MS/MS

참고문헌 (24)

참고문헌 신청

Akingbemi BT / 2001 / Modulation of rat Leydig cell steroidogenic function by di(2-ethylhexyl)phthalate / Biol Reprod 65 (4) : 1252 ~ 1259

Barr DB / 2003 / Assessing human exposure to phthalates using monoesters and their oxidized metabolites as biomarkers / Environ Health Perspect 111 (9) : 1148 ~ 1151

Duty SM / 2003 / Phthalate exposure and human semen parameters / Epidemiology 14 (3) : 269 ~ 277

Fay M / 1999 / ATSDR evaluation of health effects of chemicals. VI. Di(2-ethylhexyl)phthalate. Agency for Toxic Substances and Disease Registry / Toxicol Ind Health 15 (8) :

Fisher JS / 2003 / Human ‘testicular dysgenesis syndrome’: a possible model using inutero exposure of the rat to dibutyl phthalate / Hum Reprod 18 (7) : 1383 ~ 1394

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