지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Objectives: This study aimed to demonstrate the activities and phosphorylation changes induced by acute ethanol treatment and withdrawal conditions in the intracellular signal transduction molecules [such as extracellular signal-regulated kinase (ERK), glycogen synthase kinase 3β(GSK3β), and Akt] of the SH-SY5Y neuroblastoma cell line.
Methods: The acute treatment exposed SH-SY5Y cells to 100 mM ethanol, and we took samples 30 minutes, 60 minutes, and 24 hours after initiating this treatment. After 24 hours’ continuous ethanol treatment, we initiated ethanol withdrawal, taking samples at 30 minutes and 60 minutes.
We assayed the kinase phosphorylations via an immunoblot analysis using phosphorspecific antibodies, quantified by optical densitometry.
Results: Ethanol treatment induced a transient increase in phosphorylation of GSK3β and Akt at 30 minutes but failed to change the phosphorylation level of ERK. Ethanol withdrawal induced a transient ERK phosphorylation increase at 30 minutes, but it had no effect on the phosphorylation of GSK3β or Akt.
Conclusion: The results indicate that the ethanol-induced cellular response includes the ERK,GSK3β, and Akt systems. In particular, the ERK pathway may play a role in the acute withdrawal response. This also suggests that a relatively short exposure to ethanol, such as the 24-hour exposure in this study, can induce functional adaptation within a cell.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Ahn YM / 2005 / Increased phosphorylation of Ser473-Akt, Ser9-GSK-3beta and Ser133-CREB in the rat frontal cortex after MK-801 intraperitoneal injection / Int J Neuropsychop
Ahn YM / 2006 / The effects of MK-801 on the phosphorylation of Ser338-c-Raf-MEK-ERK pathway in the rat frontal cortex / Int J Neuropsychopharmacol 9 : 451 ~ 456
Arlinde C / 2004 / A cluster of differentially expressed signal transduction genes identified by microarray analysis in a rat genetic model of alcoholism / Pharmacogenomics
Atkins CM / 1998 / The MAPK cascade is required for mammalian associative learning / Nat Neurosci 1 : 602 ~ 609
Beaulieu JM / 2007 / Not only lithium: regulation of glycogen synthase kinase-3 by antipsychotics and serotonergic drugs / Int J Neuropsychopharmacol 10 : 3 ~ 6
Ahn YM / 2006 / The effects of MK-801 on the phosphorylation of Ser338-c-Raf-MEK-ERK pathway in the rat frontal cortex / Int J Neuropsychopharmacol 9 : 451 ~ 456
Arlinde C / 2004 / A cluster of differentially expressed signal transduction genes identified by microarray analysis in a rat genetic model of alcoholism / Pharmacogenomics
Atkins CM / 1998 / The MAPK cascade is required for mammalian associative learning / Nat Neurosci 1 : 602 ~ 609
Beaulieu JM / 2007 / Not only lithium: regulation of glycogen synthase kinase-3 by antipsychotics and serotonergic drugs / Int J Neuropsychopharmacol 10 : 3 ~ 6
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Different expression of GSK3β and pS9GSK3β depending on phenotype of cervical cancer: possible association of GSK3β with squamous cell carcinoma and pS9GSK3β with adenocarcinoma
Obstetrics & Gynecology Science
2019 .01
-
인체 모유두세포의 증식에 미치는 갈근 에탄올추출물의 효과
동의생리병리학회지
2017 .06
-
인체 모유두세포의 증식에 미치는 갈근 에탄올추출물의 효과
동의생리병리학회지
2017 .01
-
Resveratrol alleviates brain ischemic injury through the regulation of the Akt / GSK3 pathway
한국실험동물학회 학술발표대회 논문집
2018 .01
-
저칼륨혈증 흰쥐 신장에서 Akt, p-Akt, ERK 및 p-ERK 단백발현의 변화
해부·생물인류학
2017 .09
-
Interaction between Neuronal Depolarization and MK-801 in SH-SY5Y Cells and the Rat Cortex
PSYCHIATRY INVESTIGATION
2008 .01
-
Apoptosis of hippocampal neurons on Synergistic effect of amyloid β-peptide1-42 and ethanol
대한체질인류학회 학술대회 연제 초록
2006 .05
-
Simultaneous Detection of Seven Phosphoproteins in a Single Lysate Sample during Oocyte Maturation Process
대한생식의학회지
2009 .01
-
폐암세포주에서 PS-341에 의한 아포프토시스에서 JNK와 GSK-$3{\beta}$의 역할 및 상호관련성
Tuberculosis and Respiratory Diseases
2004 .01
-
홍삼수용성추출물이 혈관신생에 미치는 영향
동의생리병리학회지
2009 .01
-
Ezrin-radixin-moesin proteins are regulated by Akt-GSK3β signaling in the rat nucleus accumbens core
The Korean Journal of Physiology & Pharmacology
2020 .01
-
C6 신경아교종 세포주에서 망간에 의한 Inducible Nitric Oxide Synthase 유도
대한직업환경의학회지
2009 .09
-
Iris Nertschinskia Ethanol Extract Differentially Induces Cytotoxicity in Human Breast Cancer Cells Depending on AKT1/2 Activity
Asian Pacific journal of cancer prevention : APJCP
2012 .01
-
Ethanol Exposure Increases JNK Phosphorylation and bax Expression in Adult Rat Testes
Laboratory Animal Research
2006 .09
-
Increased Glycogen Synthase Kinase-3β mRNA Level in the Hippocampus of Patients with Major Depression: A Study Using the Stanley Neuropathology Consortium Integrative Database
PSYCHIATRY INVESTIGATION
2010 .01
-
Reactive Oxygen Species Mediate ET-1-Induced Activation of ERK1/2 Signaling in Cultured Feline Esophageal Smooth Muscle Cells
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
2007 .01
-
The Costimulation of Selenium Treatment and Selenoprotein M Overexpression Significantly Induced the Up- and Down-regulation of ERK MAPK Signaling Pathway in Various Tissues
Laboratory Animal Research
2009 .09
-
Glycogen synthase kinase 3β and cyclin D1 expression in cervical carcinogenesis
Obstetrics & Gynecology Science
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0