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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한병리학회 Journal of Pathology and Translational Medicine Journal of Pathology and Translational Medicine 제40권 제5호
- 발행연도
- 2006.1
- 수록면
- 339 - 347 (9page)
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초록· 키워드
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Background : The effect of genistein on different types of cells has been investigated. However, its effect on the nervous system is still unclear. The aim of the present work is to explore the effect of genistein on rat neuroblastoma B35 cells. Methods : The effect of genistein on the proliferation of B35 cells, its cytotoxicity, the cell-cycle distribution, the ultra-structural changes and the induction of apoptosis were determined using MTT assay, LDH assay, Flow-cytometric
analysis, transmission electron microscopy and Hoechst staining, respectively. Furthermore, Real-time quantitative RT-PCR and Western blotting were used to examine the transcriptional and post-translational alterations of the G2/M cell-cycle arrest marker cyclin-dependent kinase inhibitor p21waf1/cip1 and the apoptosis-related genes after genistein treatment. Results : Genistein
significantly inhibits cell survival, slightly elevates the release of lactate dehydrogenase
and induced apoptosis in B35 cells. Genistein increased the number of cells at S-phase and induced cells to accumulate at the G2/M phase. These G2/M arrested cells are associated with a marked up-regulation of p21waf1/cip1 at both the mRNA and protein levels. We observed that genistein up-regulates pro-apoptotic Bax with concurrent down-regulation of the anti-apoptotic Bcl-2 protein. Conclusion : These observations suggest that the anticancer effect of genistein on B35 neuroblastoma cells is mediated through multiple cellular pathways including G2/M
cell-cycle arrest and the induction of apoptosis.
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등록된 정보가 없습니다.
참고문헌 (27)
참고문헌 신청
Alhasan SA / 2000 / Genistein induced molecular changes in a squamous cell carcinoma of the head and neck cell line 16 : 333 ~ 338
Bektic J / 2005 / Molecular effects of the isoflavonoid genistein in prostate cancer 4 : 124 ~ 129
Casagrande F / 2001 / Effects of structurally related flavonoids on cell cycle progression of human melanoma cells:regulation of cyclin-dependent kinases CDK2 and CDK1 61 :
Chang HC / 2000 / Mass spe
Choi YH / 2000 / P53-independent induction of p21(WAF1/CIP1)reduction of cyclin B1 and G2/M arrest by the isoflavone genistein in human prostate carcinoma cells 91 : 164 ~ 1
Bektic J / 2005 / Molecular effects of the isoflavonoid genistein in prostate cancer 4 : 124 ~ 129
Casagrande F / 2001 / Effects of structurally related flavonoids on cell cycle progression of human melanoma cells:regulation of cyclin-dependent kinases CDK2 and CDK1 61 :
Chang HC / 2000 / Mass spe
Choi YH / 2000 / P53-independent induction of p21(WAF1/CIP1)reduction of cyclin B1 and G2/M arrest by the isoflavone genistein in human prostate carcinoma cells 91 : 164 ~ 1
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Molecular Effects of Genistein on Proliferation and Apoptosis of MCF-7 Cell Line
Molecular & cellular toxicology
2006 .01
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Preferential Cytotoxic Effect of Genistein on G361 Melanoma Cells Via Inhibition of the Expression of Focal Adhesion Kinase
International Journal of Oral Biology
2012 .01
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미성숙 암컷 흰쥐의 성 성숙에 미치는 genistein의 효과
Clinical and Experimental Pediatrics
2009 .01
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Genistein이 햄스터 난소세포의 항산화효소활성과 발현에 미치는 영향
약학회지
2007 .01
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Synthesizing efficacious genistein in conjugation with superparamagnetic Fe3O4 decorated with bio-compatible carboxymethylated chitosan against acute leukemia lymphoma
생체재료학회지
2020 .01
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Genistein이 유방암예방 활성에 미치는 영향
동의생리병리학회지
2006 .01
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Sodium Salicylate Induces the Cyclin-dependent Kinase Inhibitor p21 (Waf1/Cip1) through PI3K-related Protein Kinase-dependent p53 Activation in A549 Cells
대한의생명과학회지
2007 .06
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Effects of Genistein on the Proliferation and Differentiation in Rat Dental Pulp Cells
대한치과재료학회 학술대회
2011 .05
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Effect of Genistein on the Aryl Hydrocarbon Receptor and Cytochrome P450 1A1 in MCF-7 Human Breast Carcinoma Cells
Environmental Analysis Health and Toxicology
2006 .03
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Pharmaceutical Evaluation of Genistein-loaded Pluronic Micelles for Oral Delivery
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
2007 .01
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Genistein from Vigna angularis Extends Lifespan in Caenorhabditis elegans
Biomolecules & Therapeutics(구 응용약물학회지)
2015 .01
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Genistein이 T lymphocyte의 Apoptosis 및 복강 Macrophage의 탐식능에 미치는 영향
약학회지
2002 .01
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GENISTEIN INHIBITS $NF-_kB-DEPENDENT$ COX-2 INDUCTION IN HUMAN BREAST EPITHELIAL CELLS BY MODULATING THE ACTIVATION OF TATA-BINDING PROTEIN
대한약학회 학술대회
2002 .01
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Protective effect of genistein on radiation-induced intestinal injury in tumor bearing mice
한국실험동물학회 학술발표대회 논문집
2013 .08
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세포주기 변화에 타른 방사선 유도 암세포 사망의 조절기전
Radiation oncology journal : ROJ
2003 .01
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Genistein이 유방암예방 활성에 미치는 영향
동의생리병리학회지
2006 .02
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K562 세포주에서 Genistein에 의해 억제되는 Radiation-induced Apoptosis의 조절 유전자
Radiation oncology journal : ROJ
2001 .01
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Chemopreventive and chemotherapeutic effects of genistein, a soy isoflavone, upon cancer development and progression in preclinical animal models
Laboratory Animal Research
2014 .12
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Effects of genistein on anti-tumor activity of cisplatin in human cervical cancer cell lines
Obstetrics & Gynecology Science
2019 .01
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