The Effects of Mechanical Strain on Bone Cell Proliferation and  Recruitment Induced by Osteocytes

고성희; 이지혜; 김소희

추천

검색

자료유형: 학술저널

저자정보: 고성희 이지혜 김소희

저널정보: 대한구강생물학회 International Journal of Oral Biology International Journal of Oral Biology 제33권 제4호

발행연도: 2008.1

수록면: 179 - 186 (8page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Several lines of evidence suggest that osteocytes play a critical role in bone remodeling. Both healthy and apoptotic osteocytes can send signals to other bone surface cells such as osteoblasts, osteoclasts, osteoclast precursors, and bone lining cells through canalicular networks. Osteocytes responding to mechanical strain may also send signals to other cells. To determine the role for osteocytes and mechanical strain in bone remodeling, we examined the effects of fluid flow shear stress on osteoclast precursor cell and osteoblast proliferation and recruitment induced by osteocytes. In addition, the effects of fluid flow shear stress on osteocyte M-CSF, RANKL, and OPG mRNA expression were also examined. MLO-Y4 cells were used as an in vitro model for osteocytes, RAW 264.7 cells and MOCP-5 cells as osteoclast precursors, and 2T3 cells as osteoblasts. MLO-Y4 cells conditioned medium (Y4-CM) was collected after 24h culture. For fluid flow experiments, MLO-Y4 cells were exposed to 2 h of pulsatile fluid flow (PFF) at 2, 4, 8, 16 ± 0.6 dynes/cm ² using the Flexcell Streamer TM system. For proliferation assays, MOCP-5, RAW 264.7, and 2T3 cells were cultured with control media or 10 - 100 % Y4 CM. Cells were cultured for 3 d, and then cells were counted. RAW 264.7 and 2T3 cell migration was assayed using transwells with control media or 10 - 100 % Y4-CM. MCSF, RANKL and OPG in MLO-Y4 mRNA expression was determined by semiquantitative RT-PCR. Y4-CM increased osteoclast precursor proliferation and migration, but decreased 2T3 cell proliferation and migration. CM from MLO-Y4 cells exposed to PFF caused decreased RAW 267.4 cell proliferation and migration and 2T3 migration compared to control Y4-CM. However, Y4-CM from cells exposed to PFF had no effect on 2T3 osteoblastic cell proliferation. PFF decreased RNAKL mRNA and increased OPG mRNA in MLO-Y4 cells compared to control (without PFF). PFF had no effect on M-CSF mRNA expression in MLO-Y4 cells. These results suggest that osteocytes can regulate bone remodeling by communication with osteoclast precursors and osteoblasts and that osteocytes can communicate mechanical signals to other cells.

Several lines of evidence suggest that osteocytes play a critical role in bone remodeling. Both healthy and apoptotic osteocytes can send signals to other bone surface cells such as osteoblasts, osteoclasts, osteoclast precursors, and bone lining cells through canalicular networks. Osteocytes responding to mechanical strain may also send signals to other cells. To determine the role for osteocytes and mechanical strain in bone remodeling, we examined the effects of fluid flow shear stress on osteoclast precursor cell and osteoblast proliferation and recruitment induced by osteocytes. In addition, the effects of fluid flow shear stress on osteocyte M-CSF, RANKL, and OPG mRNA expression were also examined. MLO-Y4 cells were used as an in vitro model for osteocytes, RAW 264.7 cells and MOCP-5 cells as osteoclast precursors, and 2T3 cells as osteoblasts. MLO-Y4 cells conditioned medium (Y4-CM) was collected after 24h culture. For fluid flow experiments, MLO-Y4 cells were exposed to 2 h of pulsatile fluid flow (PFF) at 2, 4, 8, 16 ± 0.6 dynes/cm ² using the Flexcell Streamer TM system. For proliferation assays, MOCP-5, RAW 264.7, and 2T3 cells were cultured with control media or 10 - 100 % Y4 CM. Cells were cultured for 3 d, and then cells were counted. RAW 264.7 and 2T3 cell migration was assayed using transwells with control media or 10 - 100 % Y4-CM. MCSF, RANKL and OPG in MLO-Y4 mRNA expression was determined by semiquantitative RT-PCR. Y4-CM increased osteoclast precursor proliferation and migration, but decreased 2T3 cell proliferation and migration. CM from MLO-Y4 cells exposed to PFF caused decreased RAW 267.4 cell proliferation and migration and 2T3 migration compared to control Y4-CM. However, Y4-CM from cells exposed to PFF had no effect on 2T3 osteoblastic cell proliferation. PFF decreased RNAKL mRNA and increased OPG mRNA in MLO-Y4 cells compared to control (without PFF). PFF had no effect on M-CSF mRNA expression in MLO-Y4 cells. These results suggest that osteocytes can regulate bone remodeling by communication with osteoclast precursors and osteoblasts and that osteocytes can communicate mechanical signals to other cells.

#Mechanical strain #Osteocyte #Proliferation of osteoblast and osteoclast precursors #Migration of osteoblast and osteoclast precursors

참고문헌 (20)

참고문헌 신청

Ajubi NE / 1996 / Pulsating fluid flow increases prostaglandin production by cultured chicken osteocytes- A cytoskeleton-dependent process / Biochemical and Biophysical Resea

Burr DB / 2002 / Effects of biomechanical stress on bones in animals / Bone 30 (5) : 781 ~ 786

Chen W / 1998 / Generation of mouse osteoclastogenic cell lines immortalized with SV40 large T antigen / Journal of Bone and Mineral Research 13 : 1112 ~ 1123

Engsig MT / 2000 / Therkidsen B, Lund LR, Henriksen K, Lenhard T, Foged NT, Werb Z, Delaisse JM. Matrix metalloproteinase 9 and vascular endothelial growth factor are essenti

Fuller K / 1993 / Macrophage colony-stimulating factor stimulates survival and chemotactic behavior in isolated osteoclasts / JOURNAL OF EXPERIMENTAL MEDICINE 178 (5) : 1733

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

고성희

소속기관

주요연구분야 의약학 > 임상의학 > 치의학

논문수 3 이용수 6

이지혜

소속기관 대한구강생물학회

주요연구분야 의약학 > 임상의학 > 치의학

논문수 2 이용수 2

김소희

소속기관

주요연구분야 의약학 > 임상의학 > 치의학

논문수 5 이용수 4

최근 본 자료

전체보기

구분	그룹	데이터 항목
AI 학습용 데이터	원문	원문 PDF 파일
AI 학습용 데이터	원문 + 메타 (기본/상세)	원문 PDF 파일 및 서지정보 CSV
대량 구매용 데이터	B2B 구독 방식	특정 자료 한정으로 원문 접근 권한 부여
대량 구매용 데이터	URL 전달 방식	바로 PDF 뷰어를 열람할 수 있는 URL 제공

구분	그룹	데이터 항목
AI 학습용 데이터	기본 메타	발행기관명, 간행물명, 권호명, 권(vol), 호(issue), 통권, 발행연도, 발행월, 논문명, 저자명, 시작페이지, 종료페이지, 전체페이지, 상세페이지URL
상세 메타 데이터	발행기관 메타	발행기관 이명, 영문명, 창립연도, 홈페이지URL, 발행기관 소개
	간행물 메타	부제목, 간행물 유형, ISSN, ISBN, 최초발행연도, 폐간연도, 간행빈도, 발행주기, 등재사항, 이용수, 피인용수, 권호수, 논문수, 표지이미지
	논문 메타	작성 언어, 부제목, 대등제목, 목차, 키워드, 초록, 이미지, 참고문헌, 이용수, 피인용수, 논문활용도, DBpia통합주제분류, KDC분류, DDC분류, 한국연구재단분류, UCI, DOI
	저자 메타	소속기관, 소속부서, 직급, 연구분야, 연구키워드, 이용수, 피인용수, 저자 논문활용도

구분	그룹	데이터 항목
※ 결합형/맞춤형 메타 데이터는 신청 내용에 따라 다양하게 제공 가능
이용순위 정보	주제분야별 많이 이용된 논문	“인문학”에서 많이 이용된 논문 TOP100
	이용기관별 많이 이용된 논문	“중고등학교”에서 많이 이용된 논문 TOP100
	세부기관별 많이 이용된 논문	“서울대학교”에서 많이 이용된 논문 TOP100
	키워드별 많이 이용된 논문	“Chat GPT”에서 많이 이용된 논문 TOP100
키워드 정보	많이 이용된 키워드	특정기간/분야/저널 내 많이 이용된 키워드
	많이 발행된 키워드	특정기간/분야/저널 내 많이 발행된 키워드
	많이 검색된 키워드	특정기간/분야/저널 내 많이 검색된 키워드
	연구 트렌드 키워드	특정 키워드 연관 연구동향 분석 데이터 키워드

논문 기본 정보

초록· 키워드

AI 요약

연구주제

연구배경

연구방법

연구결과

주요내용

목차

참고문헌 (20)

함께 읽어보면 좋을 논문

이 논문의 저자 정보

최근 본 자료

댓글(0)