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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 209 - 214 (6page)
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Cells that have endogenous multipotent properties can be used as a starting source for the generation of induced pluripotent cells (iPSC). In addition, small molecules associated with epigenetic reprogramming are also widely used to enhance the multi- or pluripotency of such cells. Skinderived precursor cells (SKPs) are multipotent, sphereforming and embryonic neural crest-related precursor cells.
These cells can be isolated from a juvenile or adult mammalian dermis. SKPs are also an efficient starting cell source for reprogramming and the generation of iPSCs because of the high expression levels of Sox2 and Klf4 in these cells as well as their endogenous multipotency. In this study, valproic acid (VPA), a histone deacetylase (HDAC) inhibitor, was tested in the generation of iPSCs as a potential enhancer of the reprogramming potential of SKPs. SKPs were isolated from the back skins of 5-6 week old C57BL/6 X DBA/2 F1 mice.
After passage 3, the SKPs was treated with 2 mM of VPA and the quantitative real time RT-PCR was performed to quantify the expression of Oct4 and Klf4 (pluripotency specific genes), and Snai2 and Ngfr (neural crest specific genes). The results show that Oct4 and Klf4 expression was decreased by VPA treatment. However, there were no significant changes in neural crest specific gene expression following VPA treatment. Hence, although VPA is one of the most potent of the HDAC inhibitors, it does not enhance the reprogramming of multipotent skin precursor cells in mice.
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참고문헌 (24)
참고문헌 신청
Biernaskie J / 2007 / Skin-derived precursors generate myelinating Schwann cells that promote remyelination and functional recovery after contusion spinal cord injury / J Neu
Bug G / 2005 / Valproic acid stimulates proliferation and self-renewal of hematopoietic stem cells / Cancer Res 65 : 2537 ~ 2541
Buranasinsup S / 2006 / In vitro osteogenesis from human skin-derived precursor cells / Dev Growth Differ 48 : 263 ~ 269
Eminli S / 2009 / Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cells / Nat Genet 41 : 968 ~ 976
Fernandes KJ / 2008 / Multipotent skin-derived precursors: adult neural crest-related precursors with therapeutic potential / Philos Trans R Soc Lond B Biol Sci 363 : 185 ~ 1
Bug G / 2005 / Valproic acid stimulates proliferation and self-renewal of hematopoietic stem cells / Cancer Res 65 : 2537 ~ 2541
Buranasinsup S / 2006 / In vitro osteogenesis from human skin-derived precursor cells / Dev Growth Differ 48 : 263 ~ 269
Eminli S / 2009 / Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cells / Nat Genet 41 : 968 ~ 976
Fernandes KJ / 2008 / Multipotent skin-derived precursors: adult neural crest-related precursors with therapeutic potential / Philos Trans R Soc Lond B Biol Sci 363 : 185 ~ 1
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