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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 183 - 187 (5page)
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초록· 키워드
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The present study was aimed to evaluate the influence of glutaraldehyde (GA) concentration on multiple electron microscopic (EM) immunostaining using pre-embedding peroxidase and post-embedding immunogold method.
Influence of various concentrations of GA included in the fixative on immuoreactivity was assessed in the multiple immunostaining using antisera against anti-transient receptor potential vanilloid 1 (TRPV1) for peroxidase staining and anti-GABA for immunogold labeling in the rat trigeminal caudal nucleus. Anti-TRPV1 antiserum had specificity in pre-embedding peroxidase staining when tissues were fixed with fixative containing paraformaldehyde (PFA) alone.
Immunoreactivity for TRPV1 was specific in tissues fixed with fixative containing 0.5% GA at both perfusion and postfixation steps, though the immunoreactivity was weaker than in tissues fixed with fixative containing PFA alone.
Tissues fixed with fixative containing 0.5% GA at the perfusion and postfixation steps showed specific immunogold staining for GABA. The results of the present study indicate that GA concentration is critical for immunoreactivity to antigens such as TRPV1 and GABA.
This study also suggests that the appropriate GA concentration is 0.5% for multiple immunostaining with peroxidase labeling for TRPV1 and immunogold labeling for GABA.
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참고문헌 (15)
참고문헌 신청
Berod A / 1981 / Important of fixation in immunohistochemistry : Use of formaldehyde solutions at variable pH for the localization of tyrosine hydroxylase / J Histochem Cytoc
Faulk WP / 1971 / An immunocolloid method for the electron microscope / Immunochemistry 8 : 1081 ~ 1083
Kosaka T / 1986 / Use of high concentrations of glutaraldehyde for immunocytocheminstry of transmitter-synthesizing enzymes in the central nervous system / Neuroscience 18 :
Marlier L / 1991 / Serotonergic innervation of the dorsal horn of rat spinal cord : Light and electron microscopic immunocytochemical study / J Neurocytol 20 : 310 ~ 322
Ottersen OP / 1984 / Glutamate-and GABAcontaining neurons in the mouse and rat brain, as demonstrated with a new immunocytochemical technique / J Comp Neurol 229 : 374 ~ 392
Faulk WP / 1971 / An immunocolloid method for the electron microscope / Immunochemistry 8 : 1081 ~ 1083
Kosaka T / 1986 / Use of high concentrations of glutaraldehyde for immunocytocheminstry of transmitter-synthesizing enzymes in the central nervous system / Neuroscience 18 :
Marlier L / 1991 / Serotonergic innervation of the dorsal horn of rat spinal cord : Light and electron microscopic immunocytochemical study / J Neurocytol 20 : 310 ~ 322
Ottersen OP / 1984 / Glutamate-and GABAcontaining neurons in the mouse and rat brain, as demonstrated with a new immunocytochemical technique / J Comp Neurol 229 : 374 ~ 392
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