지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.4
- 수록면
- 186 - 195 (10page)
이용수
초록· 키워드
오류제보하기
Background: Notoginseng stem-leaf (NGL) ginsenosides have not been well used. To improve their utilization, the biotransformation of NGL ginsenosides was studied using ginsenosidase type-I from Aspergillus niger g.848.
Methods: NGL ginsenosides were reacted with a crude enzyme in the RAT-5D bioreactor, and the dynamic changes of multi-ginsenosides of NGL were recognized by HPLC. The reaction products were separated using a silica gel column and identified by HPLC and NMR.
Results: All the NGL ginsenosides are protopanaxadiol-type ginsenosides; the main ginsenoside contents are 27.1% Rb3, 15.7% C-Mx1, 13.8% Rc, 11.1% Fc, 7.10% Fa, 6.44% C-Mc, 5.08% Rb2, and 4.31% Rb1. In the reaction of NGL ginsenosides with crude enzyme, the main reaction of Rb3 and C-Mx1 occurred through Rb3/C-Mx1/C-Mx; when reacted for 1 h, Rb3 decreased from 27.1% to 9.82 %, C-Mx1 increased from 15.5% to 32.3%, C-Mx was produced to 6.46%, finally into C-Mx and a small amount of C-K. When reacted for 1.5 h, all the Rb1, Rd, and Gyp17 were completely reacted, and the reaction intermediate F2 was produced to 8.25%, finally into C-K. The main reaction of Rc (13.8%) occurred through Rc/C-Mc1/CMc/C-K. The enzyme barely hydrolyzed the terminal xyloside on 3-Oe or 20-O-sugar-moiety of the substrate; therefore, 9.43 g C-Mx, 6.85 g C-K, 4.50 g R7, and 4.71 g Fc (hardly separating from the substrate) were obtained from 50 g NGL ginsenosides by the crude enzyme reaction.
Conclusion: Four monomer ginsenosides were successfully produced and separated from NGL ginsenosides by the enzyme reaction.
Methods: NGL ginsenosides were reacted with a crude enzyme in the RAT-5D bioreactor, and the dynamic changes of multi-ginsenosides of NGL were recognized by HPLC. The reaction products were separated using a silica gel column and identified by HPLC and NMR.
Results: All the NGL ginsenosides are protopanaxadiol-type ginsenosides; the main ginsenoside contents are 27.1% Rb3, 15.7% C-Mx1, 13.8% Rc, 11.1% Fc, 7.10% Fa, 6.44% C-Mc, 5.08% Rb2, and 4.31% Rb1. In the reaction of NGL ginsenosides with crude enzyme, the main reaction of Rb3 and C-Mx1 occurred through Rb3/C-Mx1/C-Mx; when reacted for 1 h, Rb3 decreased from 27.1% to 9.82 %, C-Mx1 increased from 15.5% to 32.3%, C-Mx was produced to 6.46%, finally into C-Mx and a small amount of C-K. When reacted for 1.5 h, all the Rb1, Rd, and Gyp17 were completely reacted, and the reaction intermediate F2 was produced to 8.25%, finally into C-K. The main reaction of Rc (13.8%) occurred through Rc/C-Mc1/CMc/C-K. The enzyme barely hydrolyzed the terminal xyloside on 3-Oe or 20-O-sugar-moiety of the substrate; therefore, 9.43 g C-Mx, 6.85 g C-K, 4.50 g R7, and 4.71 g Fc (hardly separating from the substrate) were obtained from 50 g NGL ginsenosides by the crude enzyme reaction.
Conclusion: Four monomer ginsenosides were successfully produced and separated from NGL ginsenosides by the enzyme reaction.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (31)
참고문헌 신청
Jin FX / 2009 / Biotransformation of natural products / Chemical Industry Press :74~113
Christensen LP / 2009 / Ginsenosides chemistry, biosynthesis, analysis, and potential health effects / Adv Food Nutr Res 55:1~99
Liu CY / 2011 / Ginsenoside contents in three different ginseng / J Dalian Polytech Univ 30:79~82
Li PF / 2010 / Comparison of ginsenosides on different ginseng stem / J Anhui Agric Sci 34:13077~13079
Zhang JT / 2008 / Ginseng is the King of HerbsdGinseng chemistry, physiology activity and pharmacokinetics / Chemical Industry Press :34~44
Christensen LP / 2009 / Ginsenosides chemistry, biosynthesis, analysis, and potential health effects / Adv Food Nutr Res 55:1~99
Liu CY / 2011 / Ginsenoside contents in three different ginseng / J Dalian Polytech Univ 30:79~82
Li PF / 2010 / Comparison of ginsenosides on different ginseng stem / J Anhui Agric Sci 34:13077~13079
Zhang JT / 2008 / Ginseng is the King of HerbsdGinseng chemistry, physiology activity and pharmacokinetics / Chemical Industry Press :34~44
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Stem-leaves of Panax as a rich and sustainable source of less-polar ginsenosides: comparison of ginsenosides from Panax ginseng, American ginseng and Panax notoginseng prepared by heating and acid treatment
Journal of Ginseng Research
2021 .01
-
인삼의 열처리 과정 중 생성되는 3종의 수산화진세노사이드에 대한 연구
생약학회지(Korean Journal of Pharmacognosy)
2020 .12
-
Biotransformation of major ginsenosides in ginsenoside model culture by lactic acid bacteria
Journal of Ginseng Research
2017 .01
-
(20s) Ginsenoside Rh2 suppress colorectal cancer cell growth by Axl signal pathway in vitro and in vivo
한국실험동물학회 학술발표대회 논문집
2020 .07
-
Anti-inflammatory and Immunosuppressive Effects of Panax notoginseng
Natural Product Sciences
2019 .12
-
Non-clinical pharmacokinetic behavior of ginsenosides
Journal of Ginseng Research
2019 .06
-
Kinetic study for the optimization of ginsenoside Rg3 production by heat treatment of ginsenoside Rb1
Journal of Ginseng Research
2015 .10
-
Methods on improvements of the poor oral bioavailability of ginsenosides: Pre-processing, structural modification, drug combination, and micro- or nano- delivery system
Journal of Ginseng Research
2023 .11
-
Influence of organic acids and heat treatment on ginsenoside conversion
Journal of Ginseng Research
2018 .10
-
Variations in Ginsenosides of Raw Ginseng According to Heating Temperature and Time
Journal of Pharmacopuncture
2020 .01
-
Microbial conversion of major ginsenosides in ginseng total saponins by Platycodon grandiflorum endophytes
Journal of Ginseng Research
2016 .10
-
Effects of gut microbiota on the pharmacokinetics of protopanaxadiol ginsenosides Rd, Rg3, F2, and compound K in healthy volunteers treated orally with red ginseng
Journal of Ginseng Research
2020 .07
-
Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea
Journal of Ginseng Research
2016 .04
-
Glycosyltransformation of ginsenoside Rh2 into two novel ginsenosides using recombinant glycosyltransferase from Lactobacillus rhamnosus and its in vitro applications
Journal of Ginseng Research
2021 .01
-
Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging
Journal of Ginseng Research
2021 .11
-
Simultaneous quantification of six nonpolar ginsenosides in white ginseng by reverse-phase high-performance liquid chromatography coupled with integrated pulsed amperometric detection
Journal of Ginseng Research
2020 .07
-
Salting-out extraction of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium based on ethanol/sodium carbonate system
Journal of Ginseng Research
2020 .01
-
Effect of anti-skin disorders of ginsenosides - A Systematic Review
Journal of Ginseng Research
2023 .09
-
Inhibition of L-type Ca2+ current by ginsenoside Rd in rat ventricular myocytes
Journal of Ginseng Research
2015 .04
-
Protective effect of ginsenoside Rh3 against anticancer drug-induced apoptosis in LLC-PK1 kidney cells
Journal of Ginseng Research
2017 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0