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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.6
- 수록면
- 208 - 217 (10page)
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Background: In the present study, we identified two cytotoxic compounds from the root of Rumex crispus L. using a bioassaybased method.
Methods and Results: Compared with the other fractions, the diethyl ether (Et₂O) fraction of R. crispus root extract exhibited the strongest of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging effect [scavenging concentration 50% (SC<SUB>50</SUB>) = 63.8 ± 1.47 ㎍/㎖], nitric oxide (NO) production inhibitory effect on the mouse macrophage cell line RAW264.7 [inhibitory concentration 50% (IC50) = 60.9 ± 7.52 ㎍/㎖] and cytotoxicity effect on the human hepatoma cell line, HepG2 [lethal concentration 50% (LC<SUB>50</SUB>) = 115.4 ± 1.86 ㎍/㎖]. According to the bioassay-based method, two cytotoxic compounds were purified from the Et₂O fraction by using column chromatography and preparative high performance liquid chromatography (prep-HPLC). These two compounds were identified as parietin and chrysophanol by using nuclear magnetic resonance (NMR) and liquid chromatography quadruple time of flight mass spectrometry (LC-QTOF-MS). In addition, both parietin and chrysophanol exhibited a cytotoxicity effect on HepG2 cells, their LC<SUB>50</SUB> values were 169.1 ± 17.67 μM and 111.5 ± 6.62 μM, respectively.
Conclusions: Parietin and chrysophanol isolated from the Et₂O fraction of the R. crispus root extract showed cytotoxicity in HepG2 cell.
Methods and Results: Compared with the other fractions, the diethyl ether (Et₂O) fraction of R. crispus root extract exhibited the strongest of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging effect [scavenging concentration 50% (SC<SUB>50</SUB>) = 63.8 ± 1.47 ㎍/㎖], nitric oxide (NO) production inhibitory effect on the mouse macrophage cell line RAW264.7 [inhibitory concentration 50% (IC50) = 60.9 ± 7.52 ㎍/㎖] and cytotoxicity effect on the human hepatoma cell line, HepG2 [lethal concentration 50% (LC<SUB>50</SUB>) = 115.4 ± 1.86 ㎍/㎖]. According to the bioassay-based method, two cytotoxic compounds were purified from the Et₂O fraction by using column chromatography and preparative high performance liquid chromatography (prep-HPLC). These two compounds were identified as parietin and chrysophanol by using nuclear magnetic resonance (NMR) and liquid chromatography quadruple time of flight mass spectrometry (LC-QTOF-MS). In addition, both parietin and chrysophanol exhibited a cytotoxicity effect on HepG2 cells, their LC<SUB>50</SUB> values were 169.1 ± 17.67 μM and 111.5 ± 6.62 μM, respectively.
Conclusions: Parietin and chrysophanol isolated from the Et₂O fraction of the R. crispus root extract showed cytotoxicity in HepG2 cell.
목차
ABSTRACT
서언
재료 및 방법
결과 및 고찰
REFERENCES
참고문헌 (36)
참고문헌 신청
Ahn SH / 2001 / Risk factors for hepatocellular carcinoma in Korea / Korean Journal of Medicine 60:123~130
Amano F / 1995 / Improved detection of nitric oxide radical(NO·) production in an activated macrophage culture with a radical scavenger, carboxy PTIO, and griess reagent / FEBS letters 368:425~428
Anagnostopoulou MA / 2006 / Radical scavenging activity of various extracts and fractions of sweet orange peel(Citrus sinensis) / Food Chemistry 94:19~25
배기춘 / 2012 / 국내산 삼채 에탄올 추출물의 항염증 효과 / 대한본초학회지 27(6):55~61
백설 / 2009 / 감송향의 in vitro 항산화 및 항염증 효과 / 동의생리병리학회지 23(4):853~859
Amano F / 1995 / Improved detection of nitric oxide radical(NO·) production in an activated macrophage culture with a radical scavenger, carboxy PTIO, and griess reagent / FEBS letters 368:425~428
Anagnostopoulou MA / 2006 / Radical scavenging activity of various extracts and fractions of sweet orange peel(Citrus sinensis) / Food Chemistry 94:19~25
배기춘 / 2012 / 국내산 삼채 에탄올 추출물의 항염증 효과 / 대한본초학회지 27(6):55~61
백설 / 2009 / 감송향의 in vitro 항산화 및 항염증 효과 / 동의생리병리학회지 23(4):853~859
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