지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.12
- 수록면
- 419 - 426 (8page)
- DOI
- 10.7783/KJMCS.2019.27.6.419
이용수
이 논문의 연구 히스토리 (2)
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
Background: This study aimed to identify antioxidant compounds from the seeds of Dolichos lablab L. by bioassay-guided isolation and recrystallization.
Methods and Results: The water layer of D. lablab L. seed extract inhibits intracellular reactive oxygen species (ROS) expressing the 2’,7’-dichlorofluorescein diacetate (DCF-DA), Cu/Zn superoxide dismutase (SOD) and catalase genes, as determined by quantitative real-time PCR (qRTPCR). Two compounds were purified from the water layer of the seeds of D. lablab L. using column chromatography and prep-high performance liquid chromatography (HPLC). Using nuclear magnetic resonance (NMR) and electrospray Ionization mass spectrometry (ESI-MS), their chemical structures were identified as 5-[(2-acetyl-2,3-dihydro-1H-indazol-1-yl)carbonyl]-4,5-dihydro-3H–furan-2-one (C<SUB>14</SUB>H1<SUB>4</SUB>N₂O₄) and stachyose.
Conclusions: Two active antioxidant compounds were purified from the seed extract of D. lablab L. seed extract and the structures of these compounds were identified as C<SUB>14</SUB>H<SUB>14</SUB>O₄N₂ and stachyose.
Methods and Results: The water layer of D. lablab L. seed extract inhibits intracellular reactive oxygen species (ROS) expressing the 2’,7’-dichlorofluorescein diacetate (DCF-DA), Cu/Zn superoxide dismutase (SOD) and catalase genes, as determined by quantitative real-time PCR (qRTPCR). Two compounds were purified from the water layer of the seeds of D. lablab L. using column chromatography and prep-high performance liquid chromatography (HPLC). Using nuclear magnetic resonance (NMR) and electrospray Ionization mass spectrometry (ESI-MS), their chemical structures were identified as 5-[(2-acetyl-2,3-dihydro-1H-indazol-1-yl)carbonyl]-4,5-dihydro-3H–furan-2-one (C<SUB>14</SUB>H1<SUB>4</SUB>N₂O₄) and stachyose.
Conclusions: Two active antioxidant compounds were purified from the seed extract of D. lablab L. seed extract and the structures of these compounds were identified as C<SUB>14</SUB>H<SUB>14</SUB>O₄N₂ and stachyose.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (23)
참고문헌 신청
Al-Snafi AE / 2017 / The pharmacology and medical importance of Dolichos lablab(Lablab purpureus): A review / IOSR Journal of Pharmacy 7:22~30
Bedard K / 2007 / The NOX family of ROSgenerating NADPH oxidases: Physiology and pathophysiology / Physiological Reviews 87:245~313
Brzozowski T / 2003 / Implications of reactive oxygen species and cytokines in gastroprotection against stress-induced gastric damage by nitric oxide releasing aspirin / International Journal of Colorectal Disease 18:320~329
Cerecetto H / 2005 / Pharmacological properties of indazole derivatives:Recent developments / Mini-Reviews in Medicinal Chemistry 5:869~878
Gaikwad DD / 2015 / Synthesis of indazole motifs and their medicinal importance: An overview / European Journal of Medicinal Chemistry 90:707~731
Bedard K / 2007 / The NOX family of ROSgenerating NADPH oxidases: Physiology and pathophysiology / Physiological Reviews 87:245~313
Brzozowski T / 2003 / Implications of reactive oxygen species and cytokines in gastroprotection against stress-induced gastric damage by nitric oxide releasing aspirin / International Journal of Colorectal Disease 18:320~329
Cerecetto H / 2005 / Pharmacological properties of indazole derivatives:Recent developments / Mini-Reviews in Medicinal Chemistry 5:869~878
Gaikwad DD / 2015 / Synthesis of indazole motifs and their medicinal importance: An overview / European Journal of Medicinal Chemistry 90:707~731
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
rs2 유전자 마커를 이용한 Stachyose 저함량 콩 계통 선발
한국육종학회지
2017 .01
-
작물육종의 부가가치 향상을 위한 종자가공처리 활성화 방안
한국육종학회지
2024 .03
-
국내외 종자산업의 현황과 GSP사업의 역할
Journal of Plant Biotechnology
2015 .01
-
우리나라 종자 생산공급분야의 성과와 과제: 벼와 채소를 중심으로
한국육종학회지
2020 .01
-
종자산업법 제정 이전의 우리나라 종자관리 법령과 제도의 변천
한국육종학회지
2020 .01
-
Persistence, Viability and Emergence Rate of Canola (Brassica napus L) in Korean Soil
Weed&Turfgrass Science
2019 .12
-
Influence of Environmental Factors on Germination and Emergence of Chenopodium album Seed Heteromorphism
Weed&Turfgrass Science
2021 .06
-
Design of seed zone map for conservation plant seeds collection in Korea
한국잡초학회 별책(학술대회 초록집)
2024 .10
-
Ecophysiological Implications of Morphophysiological Dormancy (MPD) with Different Seed Maturation Time of Coreanomecon hylomeconoides Nakai, an Endemic Perennial Herb in the Korean Peninsula
한국원예학회 학술발표요지
2021 .10
-
A comparison of the characteristic properties between soybean (Glycine max [L.] Merrill) seeds with different seed coat colors
Korean Journal of Agricultural Science
2019 .12
-
원예분야의 Seed Technology 연구
한국원예학회 학술발표요지
2019 .10
-
리폭시지나아제, 쿠니츠트립신인히비터, 7S 알파다시 서브유닛 단백질이 없으며 스타키오스 함량이 낮은 대립 노란콩 품종 ‘하영’
한국육종학회지
2022 .06
-
유기종자 법제화에 대한 농업인의 의향 분석
한국유기농업학회지
2019 .01
-
미나리 종자번식 체계 확립을 위한최적 채종단계, 층적처리 및 종자 세척방법 구명
한국육종학회지
2015 .01
-
제비꽃과 남산제비꽃의 종자 휴면과 저장에 따른 발아 특성
화훼연구
2020 .01
-
Phenotypic Analysis of Seed Yield and Yield Components in Cowpea (Vigna unguiculata L., Walp)
Plant Breeding and Biotechnology
2016 .01
-
Effects of Seed Priming on Germination Enhancement of Paprika Seeds under Stress Conditions
한국원예학회 학술발표요지
2024 .11
-
Profiling and Establishing Health-Beneficial Effects of Bioactive Compounds in Germinated Alfalfa and Buckwheat Seeds
한국식품영양과학회 학술대회발표집
2021 .10
-
Recovering Viability After Cryopreservation of Lettuce Seed with Variable Seed Moisture Contents
한국원예학회 학술발표요지
2015 .10
-
Antioxidant and Antibacterial Activity of Carrot Seed Oil according to Seed Separation Grade and Extraction Methods
한국원예학회 학술발표요지
2022 .11
이 논문의 저자 정보
이 논문과 함께 이용한 논문
Antioxidant Properties and Protective Effects of Aerial Parts from Cnidium officinale Makino on Oxidative Stress-Induced Neuronal Cell Death
Na Hyun Lee
,
Dong Sun Jung
,
Jungil Hong
Preventive Nutrition and Food Science
2021
.06
도정 유무에 따른 제주산 보리의 항산화 활성 비교
강유리
,
김현정
한국식품과학회지
2021
.06
Antioxidant Activity and Effect of Tradescantia palida Methanol Extract on Viability of UVA-irradiated Human Dermal Fibroblasts
Hyun Ok Kim
,
Young Chul Kim
Quantitative Bio-Science
2021
.05
소리쟁이 뿌리로부터 세포독성물질 분리 및 정제
조용범
,
김재연
,
권남우
외 4명
한국약용작물학회지
2019
.06
Investigation of Adsorption Characteristics of Plant based Biochar for Endosulfan
Eun-Yeong Song
,
Si-Eun Kim
,
Ji-Yeon Bae
외 3명
한국농약과학회 학술발표대회 논문집
2018
.04
최근 본 자료
댓글(0)
0