지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.10
- 수록면
- 1,679 - 1,695 (17page)
- DOI
- 10.13000/JFMSE.2018.10.30.5.1679
이용수
초록· 키워드
오류제보하기
Inshore hagfish (Eptatretus burgeri) belongs to chordate and cyclostomata, so it is considered to be an important organism for the study of embryology and biological evolution. Protein kinase C (PKC) performs a wide range of biological functions regarding proliferation, apoptosis, differentiation, motility, and inflammation with cellular signal transduction. In this study, PKC beta isoforms, a member of the conventional class, were cloned. As a result, EbPKCβI and EbPKCβII showed the same sequence in conserved regions (C1, C2, C3, and C4 domain), but not in the C-terminal called the V5 domain. The ORFs of EbPKCβI and EbPKCβII were 2,007 bp and 2,004 bp, respectively. In the analysis of tissue specific expression patterns by qPCR, EbPKCβI was remarkably highly expressed in the root of the tongue and the spinal cord, while EbPKCβII was highly expressed in the gill, liver, and gut. The EbPKC βI and EbPKCβII expressed in E. coli revealed PKC activity according to both qualitative analysis and quantitative analysis.
목차
Abstract
Ⅰ. Introduction
Ⅱ. Materials and Methods
Ⅲ. Results
References
참고문헌 (17)
참고문헌 신청
[학술지(정기간행물)] - Baneyx F / 2004 / Recombinant protein folding and misfolding in Escherichia coli / Nature biotechnology 22 (11) : 1399 ~
[학술지(정기간행물)] - Blakey Slatter CA / 2005 / Expression of PKC in the developing zebrafish, Danio rerio / Journal of neurobiology 62 (4) : 425 ~ 438
[학술지(정기간행물)] - Bobeszko M / 2004 / Expression and regulation of phospholipase D isoforms in sphingosine and phorbol ester-stimulated glioma C6 cells / Biochemical and biophysical research communications 317 (3) : 689 ~ 696
[학술지(정기간행물)] - Cole TR / 2015 / Expression and purification of the N-terminal regulatory domain of protein kinase C for biophysical studies / Protein expression and purification 110 : 14 ~ 21
[학술지(정기간행물)] - Das J / 2012 / Protein Kinase C: The Drug Target One Must See / Medicinal chemistry 2 (5)
[학술지(정기간행물)] - Blakey Slatter CA / 2005 / Expression of PKC in the developing zebrafish, Danio rerio / Journal of neurobiology 62 (4) : 425 ~ 438
[학술지(정기간행물)] - Bobeszko M / 2004 / Expression and regulation of phospholipase D isoforms in sphingosine and phorbol ester-stimulated glioma C6 cells / Biochemical and biophysical research communications 317 (3) : 689 ~ 696
[학술지(정기간행물)] - Cole TR / 2015 / Expression and purification of the N-terminal regulatory domain of protein kinase C for biophysical studies / Protein expression and purification 110 : 14 ~ 21
[학술지(정기간행물)] - Das J / 2012 / Protein Kinase C: The Drug Target One Must See / Medicinal chemistry 2 (5)
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UCI(KEPA) : I410-ECN-0101-2018-454-003547969