지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.45 No.2
- 발행연도
- 2018.6
- 수록면
- 248 - 257 (10page)
이용수
초록· 키워드
오류제보하기
Overexpression of AtCYP78A7, a gene encoding a cytochrome P450 protein, has been reported to improve tolerance to drought stress in genetically modified (GM) rice (Oryza sativa L.). The aim of this study was to evaluate the potential allergenicity and acute oral toxicity of the AtCYP78A7 protein expressed in GM rice. Bioinformatics analysis of the amino acid sequence of AtCYP78A7 did not identify any similarities with any known allergens or toxins. It showed that no known allergen had more than a 35% amino acid sequence homology with the AtCYP78A7 protein over an 80 amino acid window or more than 8 consecutive identical amino acids. The gene encoding the AtCYP78A7 protein was cloned in the pGEX-4T-1 vector and expressed in E. coli . Then, the AtCYP78A7 protein was purified and analyzed for acute oral toxicity. The AtCYP78A7 protein was fed at a dose of 2,000 ㎎/㎏ body weight in mice, and the changes in mortalities, clinical findings, and body weight were monitored for 14 days after the dosing. Necropsy was carried out on day 14. The protein did not cause any adverse effects when it was orally administered to mice at 2000 ㎎/㎏ body weight. These results indicate that the AtCYP78A7 protein expressed in GM rice would not be a potential allergen or toxin.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusions
References
참고문헌 (24)
참고문헌 신청
Codex Alimentarious Commission / 2009 / Foods derived from modern biotechnology / WHO, FAO
Delaney B / 2008 / Evaluation of protein safety in the context of agricultural biotechnology / Food and Chemical Toxicology 46:71~97
FAO/WHO / 2001 / Evaluation of allergenicity of genetically modified foods / Food and Agriculture Organization of the United Nations
Ju Sung Im / 2012 / Growth, quality, and yield characteristics of transgenic potato (Solanum tuberosum L.) overexpressing StMyb1R-1 under water deficit / Journal of Plant Biotechnology 39(3):154~162
ISAAA / 2016 / Global status of commercialized biotech/GM crops: 2016
Delaney B / 2008 / Evaluation of protein safety in the context of agricultural biotechnology / Food and Chemical Toxicology 46:71~97
FAO/WHO / 2001 / Evaluation of allergenicity of genetically modified foods / Food and Agriculture Organization of the United Nations
Ju Sung Im / 2012 / Growth, quality, and yield characteristics of transgenic potato (Solanum tuberosum L.) overexpressing StMyb1R-1 under water deficit / Journal of Plant Biotechnology 39(3):154~162
ISAAA / 2016 / Global status of commercialized biotech/GM crops: 2016
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UCI(KEPA) : I410-ECN-0101-2018-520-003133100