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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.10
- 수록면
- 406 - 413 (8page)
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이 논문의 연구 히스토리 (2)
2015
Leaf-specific pathogenesis-related 10 homolog, PgPR-10.3, shows in silico binding affinity with several biologically important molecules
Jin Haeng Han
,
Jin Hee Lee
,
Ok Ran Lee
Journal of Ginseng Research
2015.10
학술저널
Leaf-specific pathogenesis-related 10 homolog, PgPR-10.3, shows in silico binding affinity with several biologically important molecules
Jin Haeng Han
,
Jin Hee Lee
,
Ok Ran Lee
한국약용작물학회 학술대회논문집
2015.05
학술대회자료
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초록· 키워드
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Background: Pathogenesis-related 10 (PR-10) proteins are small, cytosolic proteins with a similar three-dimensional structure. Crystal structures for several PR-10 homologs have similar overall folding patterns, with an unusually large internal cavity that is a binding site for biologically important molecules. Although structural information on PR-10 proteins is substantial, understanding of their biological function remains limited. Here, we showed that one of the PgPR-10 homologs, PgPR-10.3, shares binding properties with flavonoids, kinetin, emodin, deoxycholic acid, and ginsenoside Re (1 of the steroid glycosides).
Methods: Gene expression patterns of PgPR-10.3 were analyzed by quantitative real-time PCR. The three-dimensional structure of PgPR-10 proteins was visualized by homology modeling, and docking to retrieve biologically active molecules was performed using AutoDock4 program.
Results: Transcript levels of PgPR-10.3 expressed in leaves, stems, and roots of 3-wk-old ginseng plantlets were on average 86-fold lower than those of PgPR-10.2. In mature 2-yr-old ginseng plants, the mRNA of PgPR-10.3 is restricted to leaves. Ginsenoside Re production is especially prominent in leaves of Panax ginseng Meyer, and the binding property of PgPR-10.3 with ginsenoside Re suggests that this protein has an important role in the control of secondary metabolism.
Conclusion: Although ginseng PR-10.3 gene is expressed in all organs of 3-wk-old plantlets, its expression is restricted to leaves in mature 2-yr-old ginseng plants. The putative binding property of PgPR-10.3 with Re is intriguing. Further verification of binding affinity with other biologically important molecules in the large hydrophobic cavity of PgPR-10.3 may provide an insight into the biological features of PR-10 proteins.
Methods: Gene expression patterns of PgPR-10.3 were analyzed by quantitative real-time PCR. The three-dimensional structure of PgPR-10 proteins was visualized by homology modeling, and docking to retrieve biologically active molecules was performed using AutoDock4 program.
Results: Transcript levels of PgPR-10.3 expressed in leaves, stems, and roots of 3-wk-old ginseng plantlets were on average 86-fold lower than those of PgPR-10.2. In mature 2-yr-old ginseng plants, the mRNA of PgPR-10.3 is restricted to leaves. Ginsenoside Re production is especially prominent in leaves of Panax ginseng Meyer, and the binding property of PgPR-10.3 with ginsenoside Re suggests that this protein has an important role in the control of secondary metabolism.
Conclusion: Although ginseng PR-10.3 gene is expressed in all organs of 3-wk-old plantlets, its expression is restricted to leaves in mature 2-yr-old ginseng plants. The putative binding property of PgPR-10.3 with Re is intriguing. Further verification of binding affinity with other biologically important molecules in the large hydrophobic cavity of PgPR-10.3 may provide an insight into the biological features of PR-10 proteins.
목차
abstract
1. Introduction
2. Materials and methods
3. Results and discussion
4. Conclusion
References
참고문헌 (26)
참고문헌 신청
[학술지(정기간행물)] - Pulla RK / 2010 / Expression and functional characterization of pathogenesis-related protein family 10 gene, PgPR10-2, from Panax ginseng C.A. Meyer / Physiol Mol Plant Pathol 74 : 323 ~ 329
[학술지(정기간행물)] - 이옥란 / 2011 / Defense Genes Induced by Pathogens and Abiotic Stresses in Panax ginseng C. A. Meyer / Journal of Ginseng Research 35 (1) : 1 ~ 11
[학술지(정기간행물)] - Lee OR / 2012 / Expression of the ginseng PgPR10-1 in Arabidopsis confers resistance against fungal and bacterial infection / Gene 506 : 85 ~ 95
[학술지(정기간행물)] - Lee OR / 2012 / Expression and stress tolerance of PR10 genes from Panax ginseng C.A. Meyer / Mol Biol Rep 39 : 2365 ~ 2374
[학술지(정기간행물)] - Gajhede M / 1996 / X-ray and NMR structure of Bet v 1, the origin of birch pollen allergy / Nat Struct Biol 3 : 1040 ~ 1045
[학술지(정기간행물)] - 이옥란 / 2011 / Defense Genes Induced by Pathogens and Abiotic Stresses in Panax ginseng C. A. Meyer / Journal of Ginseng Research 35 (1) : 1 ~ 11
[학술지(정기간행물)] - Lee OR / 2012 / Expression of the ginseng PgPR10-1 in Arabidopsis confers resistance against fungal and bacterial infection / Gene 506 : 85 ~ 95
[학술지(정기간행물)] - Lee OR / 2012 / Expression and stress tolerance of PR10 genes from Panax ginseng C.A. Meyer / Mol Biol Rep 39 : 2365 ~ 2374
[학술지(정기간행물)] - Gajhede M / 1996 / X-ray and NMR structure of Bet v 1, the origin of birch pollen allergy / Nat Struct Biol 3 : 1040 ~ 1045
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UCI(KEPA) : I410-ECN-0101-2018-524-001598012