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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.4
- 수록면
- 113 - 120 (8page)
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초록· 키워드
오류제보하기
Background: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level.
Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods.
Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng.
Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng.
Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods.
Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng.
Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
5. Conclusion
References
참고문헌 (49)
참고문헌 신청
[학술지(정기간행물)] - Choi YE / 2006 / Ginseng (Panax ginseng) / Methods Mol Biol 344 : 361 ~ 371
[학술지(정기간행물)] - Attele AS / 1999 / Ginseng pharmacology: multiple constituents and multiple actions / Biochem Pharmacol 58 : 1685 ~ 1693
[학술지(정기간행물)] - Wang J / 2010 / Anti-fatigue activity of the water-soluble polysaccharides isolated from Panax ginseng C. A. Meyer / J Ethnopharmacol 130 : 421 ~ 423
[단행본] - Wang TS / 2001 / Chinese ginseng. Shenyang (LN) / Liaoning Scientific and Technical Publishers
[학술지(정기간행물)] - Qi LW / 2011 / Isolation and analysis of ginseng: advances and challenges / Nat Prod Rep 28 : 467 ~ 495
[학술지(정기간행물)] - Attele AS / 1999 / Ginseng pharmacology: multiple constituents and multiple actions / Biochem Pharmacol 58 : 1685 ~ 1693
[학술지(정기간행물)] - Wang J / 2010 / Anti-fatigue activity of the water-soluble polysaccharides isolated from Panax ginseng C. A. Meyer / J Ethnopharmacol 130 : 421 ~ 423
[단행본] - Wang TS / 2001 / Chinese ginseng. Shenyang (LN) / Liaoning Scientific and Technical Publishers
[학술지(정기간행물)] - Qi LW / 2011 / Isolation and analysis of ginseng: advances and challenges / Nat Prod Rep 28 : 467 ~ 495
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UCI(KEPA) : I410-ECN-0101-2018-524-001598178