지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.4
- 수록면
- 169 - 179 (11page)
이용수
초록· 키워드
오류제보하기
Background: Ginsenosides are the main pharmacological components of Panax ginseng root, which are thought to be primarily responsible for the suppressing effect on oxidative stress.
Methods: 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorption capacity were applied to evaluate the antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293) cells were incubated with ginsenosides extracted by pulsed electric field (PEF) and solvent cold soak extraction (SCSE) for 24 h and then the injury was induced by 40μM H₂O₂. The cell viability and surface morphology of HEK-293 cells were studied using MTS assay and scanning electron microscopy, respectively. Dichloro-dihydro-fluorescein diacetate fluorescent probe assay was used to measure the level of intracellular reactive oxygen species. The intracellular antioxidant activities of ginsenosides were evaluated by cellular antioxidant activity assay in HepG2 cells.
Results: The PEF extracts displayed the higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and stronger oxygen radical absorption capacity (with an oxygen radical absorption capacity value of 14.48 ± 4.04μM TE per μg/mL). The HEK-293 cell model also suggested that the protective effect of PEF extracts was dose-dependently greater than SCSE extracts. Dichloro-dihydro-fluorescein diacetate assay further proved that PEF extracts are more active (8% higher than SCSE extracts) in reducing intracellular reactive oxygen species accumulation. In addition, scanning electron microscopy images showed that the HEK-293 cells, which were treated with PEF extracts, maintained more intact surface morphology. Cellular antioxidant activity values indicated that ginsenosides extracted by PEF had stronger cellular antioxidant activity than SCSE ginsenosides extracts.
Conclusion: The present study demonstrated the antioxidative effect of ginsenosides extracted by PEF in vitro. Furthermore, rather than SCSE, PEF may be more useful as an alternative extraction technique for the extraction of ginsenosides with enhanced antioxidant activity.
Methods: 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorption capacity were applied to evaluate the antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293) cells were incubated with ginsenosides extracted by pulsed electric field (PEF) and solvent cold soak extraction (SCSE) for 24 h and then the injury was induced by 40μM H₂O₂. The cell viability and surface morphology of HEK-293 cells were studied using MTS assay and scanning electron microscopy, respectively. Dichloro-dihydro-fluorescein diacetate fluorescent probe assay was used to measure the level of intracellular reactive oxygen species. The intracellular antioxidant activities of ginsenosides were evaluated by cellular antioxidant activity assay in HepG2 cells.
Results: The PEF extracts displayed the higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and stronger oxygen radical absorption capacity (with an oxygen radical absorption capacity value of 14.48 ± 4.04μM TE per μg/mL). The HEK-293 cell model also suggested that the protective effect of PEF extracts was dose-dependently greater than SCSE extracts. Dichloro-dihydro-fluorescein diacetate assay further proved that PEF extracts are more active (8% higher than SCSE extracts) in reducing intracellular reactive oxygen species accumulation. In addition, scanning electron microscopy images showed that the HEK-293 cells, which were treated with PEF extracts, maintained more intact surface morphology. Cellular antioxidant activity values indicated that ginsenosides extracted by PEF had stronger cellular antioxidant activity than SCSE ginsenosides extracts.
Conclusion: The present study demonstrated the antioxidative effect of ginsenosides extracted by PEF in vitro. Furthermore, rather than SCSE, PEF may be more useful as an alternative extraction technique for the extraction of ginsenosides with enhanced antioxidant activity.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
4. Conclusion
References
참고문헌 (47)
참고문헌 신청
[학술지(정기간행물)] - Pyo CW / 2013 / Oxidative stress-induced cyclin d1 depletion and its role in cell cycle processing / Biochim Biophys Acta 1830 : 5316 ~ 5325
[학술지(정기간행물)] - Samuni Y / 2013 / The chemistry and biological activities of N-acetylcysteine / Biochim Biophys Acta 1830 : 4117 ~ 4129
[학술지(정기간행물)] - Zhou W / 2004 / Molecular mechanisms and clinical applications of ginseng root for cardiovascular disease / Med Sci Monit 10 : RA187 ~ RA192
[학술지(정기간행물)] - Lu JM / 2012 / Ginsenoside rb1 directly scavenges hydroxyl radical and hypochlorous acid / Curr Pharm Des 18 : 6339 ~ 6347
[학술지(정기간행물)] - Ni N / 2014 / Ginsenoside rb1 protects rat neural progenitor cells against oxidative injury / Molecules 19 : 3012 ~ 3024
[학술지(정기간행물)] - Samuni Y / 2013 / The chemistry and biological activities of N-acetylcysteine / Biochim Biophys Acta 1830 : 4117 ~ 4129
[학술지(정기간행물)] - Zhou W / 2004 / Molecular mechanisms and clinical applications of ginseng root for cardiovascular disease / Med Sci Monit 10 : RA187 ~ RA192
[학술지(정기간행물)] - Lu JM / 2012 / Ginsenoside rb1 directly scavenges hydroxyl radical and hypochlorous acid / Curr Pharm Des 18 : 6339 ~ 6347
[학술지(정기간행물)] - Ni N / 2014 / Ginsenoside rb1 protects rat neural progenitor cells against oxidative injury / Molecules 19 : 3012 ~ 3024
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UCI(KEPA) : I410-ECN-0101-2018-524-001598825