지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.6
- 수록면
- 186 - 194 (9page)
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Various human host cell lines, which are more effective than the other original human cell lines, have been developed and used. Highly efficient human cell line can be obtained from the fusion between human embryonic kidney 293 (HEK293) and human Burkitt’s lymphoma cells (Namalwa). Fused cell line has the advantages of both cell lines such as the high transfection efficacy of HEK293 cells and the constitutive expression of Epstein-Barr virus (EBV) genome which is related with high expression of target protein and anti-apoptotic growth of Namalwa cells. In this study, characterization of two original cell lines was performed by using design of experiment (DOE) considering cell maintenance, media development, optimization of culture condition, and scale-up. The formation of aggregates was apparent with high glutamine concentration at more than 6 mM. Supplementation of hydrolysates showed positive effects on the growth performances of HEK293 cells. On the contrary, Namalwa cells showed negative results. It was confirmed that Namalwa cells were more sensitive to lower temperature at 35℃ and hyperosmotic condition over 260 mOsm/kg. In addition, both cell lines showed limited growth in 3-L bioreactor due to shear stress.
목차
Abstract
1. 서론
2. 재료 및 방법
3. 결과 및 고찰
4. 결론
References
참고문헌 (23)
참고문헌 신청
1. [학술지(정기간행물)] - Butler, M / 2005 / Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals / Appl. Microbiol. Biotechnol 68 : 283 ~ 291
2. [학술지(정기간행물)] - Cho, M. S / 2002 / Establishment of a human somatic hybrid cell line for recombinant protein production / J. Biomed. Sci 9 : 631 ~ 638
3. [학술지(정기간행물)] - Cho, M. S / 2003 / Versatile expression system for rapid and stable production of recombinant proteins / Biotechnol. Prog 19 : 229 ~ 232
4. [학술지(정기간행물)] - Mei, B / 2006 / Expression of human coagulation factor VIII in a human hybrid cell line, HKB11 / Mol. Biotechnol 34 : 165 ~ 178
5. [학술지(정기간행물)] - Klein, G / 2010 / Interaction of epsteinbarr virus (EBV) with human B-lymphocytes / Biochem. Biophys. Res. Commun 396 : 67 ~ 73
2. [학술지(정기간행물)] - Cho, M. S / 2002 / Establishment of a human somatic hybrid cell line for recombinant protein production / J. Biomed. Sci 9 : 631 ~ 638
3. [학술지(정기간행물)] - Cho, M. S / 2003 / Versatile expression system for rapid and stable production of recombinant proteins / Biotechnol. Prog 19 : 229 ~ 232
4. [학술지(정기간행물)] - Mei, B / 2006 / Expression of human coagulation factor VIII in a human hybrid cell line, HKB11 / Mol. Biotechnol 34 : 165 ~ 178
5. [학술지(정기간행물)] - Klein, G / 2010 / Interaction of epsteinbarr virus (EBV) with human B-lymphocytes / Biochem. Biophys. Res. Commun 396 : 67 ~ 73
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